Peptidyl-tRNA. IX. The use of o-nitrophenylsulfenyl group as N-protecting group in the synthesis of peptidyl-tRNA

Y. Lapidot; D. Elat; S. Rappoport; N. de Groot

doi:10.1016/0006-291X(70)90617-0

Peptidyl-tRNA. IX. The use of o-nitrophenylsulfenyl group as N-protecting group in the synthesis of peptidyl-tRNA

Y. Lapidot^*
, D. Elat
, S. Rappoport
, N. de Groot

^*Corresponding author for this work

Alexander Silberman Institute of Life Sciences

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The use of o-nitrophenylsulfenyl group (NPS) as N-blocking group in the synthesis of peptidyl-tRNA is demonstrated by the preparation of val-gly-[¹⁴C]phe-tRNA. The synthesis involves a reaction between N-hydroxysuccinimide ester of NPS-val-gly and [¹⁴C]phe-tRNA. The NPS group is removed from the N-blocked peptidyl-tRNA by treatment with 0.2 M sodium thiosulfate at 30° C for 2 hrs.

Original language	English
Pages (from-to)	559-563
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	38
Issue number	4
DOIs	https://doi.org/10.1016/0006-291X(70)90617-0
State	Published - 20 Feb 1970

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title = "Peptidyl-tRNA. IX. The use of o-nitrophenylsulfenyl group as N-protecting group in the synthesis of peptidyl-tRNA",

abstract = "The use of o-nitrophenylsulfenyl group (NPS) as N-blocking group in the synthesis of peptidyl-tRNA is demonstrated by the preparation of val-gly-[14C]phe-tRNA. The synthesis involves a reaction between N-hydroxysuccinimide ester of NPS-val-gly and [14C]phe-tRNA. The NPS group is removed from the N-blocked peptidyl-tRNA by treatment with 0.2 M sodium thiosulfate at 30° C for 2 hrs.",

author = "Y. Lapidot and D. Elat and S. Rappoport and \{de Groot\}, N.",

year = "1970",

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AU - Elat, D.

AU - Rappoport, S.

AU - de Groot, N.

PY - 1970/2/20

Y1 - 1970/2/20

N2 - The use of o-nitrophenylsulfenyl group (NPS) as N-blocking group in the synthesis of peptidyl-tRNA is demonstrated by the preparation of val-gly-[14C]phe-tRNA. The synthesis involves a reaction between N-hydroxysuccinimide ester of NPS-val-gly and [14C]phe-tRNA. The NPS group is removed from the N-blocked peptidyl-tRNA by treatment with 0.2 M sodium thiosulfate at 30° C for 2 hrs.

AB - The use of o-nitrophenylsulfenyl group (NPS) as N-blocking group in the synthesis of peptidyl-tRNA is demonstrated by the preparation of val-gly-[14C]phe-tRNA. The synthesis involves a reaction between N-hydroxysuccinimide ester of NPS-val-gly and [14C]phe-tRNA. The NPS group is removed from the N-blocked peptidyl-tRNA by treatment with 0.2 M sodium thiosulfate at 30° C for 2 hrs.

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JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Peptidyl-tRNA. IX. The use of o-nitrophenylsulfenyl group as N-protecting group in the synthesis of peptidyl-tRNA

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