Peptidyl-tRNA. XI. The chemical synthesis of phenylalanine-containing oligopeptidyl-tRNA

A. D. Hamburger; N. De Groot; Y. Lapidot

doi:10.1016/0005-2787(70)90012-2

Peptidyl-tRNA. XI. The chemical synthesis of phenylalanine-containing oligopeptidyl-tRNA

A. D. Hamburger^*, N. De Groot, Y. Lapidot

^*Corresponding author for this work

Alexander Silberman Institute of Life Sciences

Research output: Contribution to journal › Article › peer-review

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Abstract

The synthesis of phenylalanine-containing peptidyl-tRNA is described. The method involves a reaction between the N-hydroxysuccinimide ester of N-o-nitrophenylsulfenyl-l-Phe_n (n = 1-2) or N-o-nitrophenylsulfenyl-l-Phe_n-Gly (n = 1-3) and aminoacyl-tRNA and subsequent removal of the N-protecting group by treatment with Na₂S₂O₃ in the presence of 6 M urea. Phe-Phe-[¹⁴C]Phe-tRNA was also prepared by the reaction between the N-hydroxysuccinimide ester of N-monomethoxytrityl-Phe-Phe and [¹⁴C]Phe-tRNA. The monomethoxytrityl group was removed from the blocked peptidyl-tRNA under mild acidic conditions (5% dichloroacetic acid at 4°).

Original language	English
Pages (from-to)	115-123
Number of pages	9
Journal	BBA Section Nucleic Acids And Protein Synthesis
Volume	213
Issue number	1
DOIs	https://doi.org/10.1016/0005-2787(70)90012-2
State	Published - 16 Jul 1970

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title = "Peptidyl-tRNA. XI. The chemical synthesis of phenylalanine-containing oligopeptidyl-tRNA",

abstract = "The synthesis of phenylalanine-containing peptidyl-tRNA is described. The method involves a reaction between the N-hydroxysuccinimide ester of N-o-nitrophenylsulfenyl-l-Phen (n = 1-2) or N-o-nitrophenylsulfenyl-l-Phen-Gly (n = 1-3) and aminoacyl-tRNA and subsequent removal of the N-protecting group by treatment with Na2S2O3 in the presence of 6 M urea. Phe-Phe-[14C]Phe-tRNA was also prepared by the reaction between the N-hydroxysuccinimide ester of N-monomethoxytrityl-Phe-Phe and [14C]Phe-tRNA. The monomethoxytrityl group was removed from the blocked peptidyl-tRNA under mild acidic conditions (5% dichloroacetic acid at 4°).",

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N2 - The synthesis of phenylalanine-containing peptidyl-tRNA is described. The method involves a reaction between the N-hydroxysuccinimide ester of N-o-nitrophenylsulfenyl-l-Phen (n = 1-2) or N-o-nitrophenylsulfenyl-l-Phen-Gly (n = 1-3) and aminoacyl-tRNA and subsequent removal of the N-protecting group by treatment with Na2S2O3 in the presence of 6 M urea. Phe-Phe-[14C]Phe-tRNA was also prepared by the reaction between the N-hydroxysuccinimide ester of N-monomethoxytrityl-Phe-Phe and [14C]Phe-tRNA. The monomethoxytrityl group was removed from the blocked peptidyl-tRNA under mild acidic conditions (5% dichloroacetic acid at 4°).

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Peptidyl-tRNA. XI. The chemical synthesis of phenylalanine-containing oligopeptidyl-tRNA

Abstract

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