Peptidyl-tRNA. XII. Behaviour of peptidyl-tRNAPhe from Escherichia coli on methylated albumin silicic acid column

E. Ziv; N. De Groot; Y. Lapidot

doi:10.1016/0005-2787(71)90553-3

Peptidyl-tRNA. XII. Behaviour of peptidyl-tRNA^Phe from Escherichia coli on methylated albumin silicic acid column

E. Ziv^*
, N. De Groot
, Y. Lapidot

^*Corresponding author for this work

Alexander Silberman Institute of Life Sciences

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The behaviour of different peptidyl-tRNA^Phe from Escherichia coli on methylated albumin silicic acid column was studied. It was found that the behaviour of glycine, alanine and serine containing peptidyl-tRNA^Phe on this column was similar to that of Phe-tRNA. The elution pattern of N-blocked peptidyl-tRNA, on the other hand, differed from that of Phe-tRNA or unblocked peptidyl-tRNA. Phe-Gly-Phe-tRNA was strongly retarded on the methylated albumin silicic acid column as compared with Phe-tRNA. It is suggested that this retardation is due to the hydrophobic properties of the phenylalanine.

Original language	English
Pages (from-to)	135-140
Number of pages	6
Journal	BBA Section Nucleic Acids And Protein Synthesis
Volume	228
Issue number	1
DOIs	https://doi.org/10.1016/0005-2787(71)90553-3
State	Published - 1 Jan 1971

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title = "Peptidyl-tRNA. XII. Behaviour of peptidyl-tRNAPhe from Escherichia coli on methylated albumin silicic acid column",

abstract = "The behaviour of different peptidyl-tRNAPhe from Escherichia coli on methylated albumin silicic acid column was studied. It was found that the behaviour of glycine, alanine and serine containing peptidyl-tRNAPhe on this column was similar to that of Phe-tRNA. The elution pattern of N-blocked peptidyl-tRNA, on the other hand, differed from that of Phe-tRNA or unblocked peptidyl-tRNA. Phe-Gly-Phe-tRNA was strongly retarded on the methylated albumin silicic acid column as compared with Phe-tRNA. It is suggested that this retardation is due to the hydrophobic properties of the phenylalanine.",

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AU - De Groot, N.

AU - Lapidot, Y.

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N2 - The behaviour of different peptidyl-tRNAPhe from Escherichia coli on methylated albumin silicic acid column was studied. It was found that the behaviour of glycine, alanine and serine containing peptidyl-tRNAPhe on this column was similar to that of Phe-tRNA. The elution pattern of N-blocked peptidyl-tRNA, on the other hand, differed from that of Phe-tRNA or unblocked peptidyl-tRNA. Phe-Gly-Phe-tRNA was strongly retarded on the methylated albumin silicic acid column as compared with Phe-tRNA. It is suggested that this retardation is due to the hydrophobic properties of the phenylalanine.

AB - The behaviour of different peptidyl-tRNAPhe from Escherichia coli on methylated albumin silicic acid column was studied. It was found that the behaviour of glycine, alanine and serine containing peptidyl-tRNAPhe on this column was similar to that of Phe-tRNA. The elution pattern of N-blocked peptidyl-tRNA, on the other hand, differed from that of Phe-tRNA or unblocked peptidyl-tRNA. Phe-Gly-Phe-tRNA was strongly retarded on the methylated albumin silicic acid column as compared with Phe-tRNA. It is suggested that this retardation is due to the hydrophobic properties of the phenylalanine.

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Peptidyl-tRNA. XII. Behaviour of peptidyl-tRNA^Phe from Escherichia coli on methylated albumin silicic acid column

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