Persistent expression of BMP-4 in embryonic chick adrenal cortical cells and its role in chromaffin cell development

Katrin Huber, Aylin Franke, Barbara Brühl, Shlomi Krispin, Uwe Ernsberger, Andreas Schober, Oliver Von Bohlen Und Halbach, Hermann Rohrer, Chaya Kalcheim, Klaus Unsicker*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


Background. Adrenal chromaffin cells and sympathetic neurons both originate from the neural crest, yet signals that trigger chromaffin development remain elusive. Bone morphogenetic proteins (BMPs) emanating from the dorsal aorta are important signals for the induction of a sympathoadrenal catecholaminergic cell fate. Results. We report here that BMP-4 is also expressed by adrenal cortical cells throughout chick embryonic development, suggesting a putative role in chromaffin cell development. Moreover, bone morphogenetic protein receptor IA is expressed by both cortical and chromaffin cells. Inhibiting BMP-4 with noggin prevents the increase in the number of tyrosine hydroxylase positive cells in adrenal explants without affecting cell proliferation. Hence, adrenal BMP-4 is likely to induce tyrosine hydroxylase in sympathoadrenal progenitors. To investigate whether persistent BMP-4 exposure is able to induce chromaffin traits in sympathetic ganglia, we locally grafted BMP-4 overexpressing cells next to sympathetic ganglia. Embryonic day 8 chick sympathetic ganglia, in addition to principal neurons, contain about 25% chromaffin-like cells. Ectopic BMP-4 did not increase this proportion, yet numbers and sizes of 'chromaffin' granules were significantly increased. Conclusion. BMP-4 may serve to promote specific chromaffin traits, but is not sufficient to convert sympathetic neurons into a chromaffin phenotype.

Original languageAmerican English
Article number28
JournalNeural Development
Issue number1
StatePublished - 2008

Bibliographical note

Funding Information:
We thank Nicole Karch and Jutta Fey for excellent technical assistance. We are grateful to Richard Sparla for providing the RT-PCR data. This work was supported by a grant from the Deutsche Forschungsgemeinschaft (SFB 488, TP A6).


Dive into the research topics of 'Persistent expression of BMP-4 in embryonic chick adrenal cortical cells and its role in chromaffin cell development'. Together they form a unique fingerprint.

Cite this