Petunia × hybrida floral scent production is negatively affected by high-temperature growth conditions

Alon Cna'Ani*, Joelle K. Mühlemann, Jasmin Ravid, Tania Masci, Antje Klempien, Thuong T.H. Nguyen, Natalia Dudareva, Eran Pichersky, Alexander Vainstein

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

Increasing temperatures due to changing global climate are interfering with plant-pollinator mutualism, an interaction facilitated mainly by floral colour and scent. Gas chromatography-mass spectroscopy analyses revealed that increasing ambient temperature leads to a decrease in phenylpropanoid-based floral scent production in two Petunia×hybrida varieties, P720 and Blue Spark, acclimated at 22/16 or 28/22°C (day/night). This decrease could be attributed to down-regulation of scent-related structural gene expression from both phenylpropanoid and shikimate pathways, and up-regulation of a negative regulator of scent production, emission of benzenoids V (EOBV). To test whether the negative effect of increased temperature on scent production can be reduced in flowers with enhanced metabolic flow in the phenylpropanoid pathway, we analysed floral volatile production by transgenic 'Blue Spark' plants overexpressing CaMV 35S-driven Arabidopsis thaliana production of anthocyanin pigments 1 (PAP1) under elevated versus standard temperature conditions. Flowers of 35S:PAP1 transgenic plants produced the same or even higher levels of volatiles when exposed to a long-term high-temperature regime. This phenotype was also evident when analysing relevant gene expression as inferred from sequencing the transcriptome of 35S:PAP1 transgenic flowers under the two temperature regimes. Thus, up-regulation of transcription might negate the adverse effects of temperature on scent production. We demonstrate that petunia flowers produce less volatile phenylpropanoid compounds, in both scent bouquets and internal pools, in response to elevated temperatures. We reveal that the decrease in floral scent is correlated with reduced transcript levels of scent-related genes, and that the adverse effect of high temperature can be negated by expressing transcriptional up-regulators. We believe that the conclusions and implications drawn from the original data presented in our manuscript will be of particular interest to a broad spectrum of your readers, particularly in view of recent changes in global climate and the risk of environmental disruption of plant-pollinator mutualism.

Original languageAmerican English
Pages (from-to)1333-1346
Number of pages14
JournalPlant, Cell and Environment
Volume38
Issue number7
DOIs
StatePublished - 1 Jul 2015

Bibliographical note

Publisher Copyright:
© 2014 John Wiley & Sons Ltd.

Keywords

  • Anthocyanin
  • Environmental stimulus
  • Petunia
  • Phenylpropanoid
  • Production of anthocyanin pigment1 (PAP1)
  • Temperature
  • Volatile

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