PF-4708671 activates AMPK independently of p70S6K1 inhibition

Gilad W. Vainer, Ann Saada, Juliane Kania-Almog, Adir Amartely, Jacob Bar-Tana, Rachel Hertz

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The P70 ribosomal protein S6 kinase 1 (P70S6K1) is activated by the mammalian target of rapamycin (mTORC1) and regulates proliferation, growth, and metabolism. PF-4708671 is a novel, cell-permeable, has been proposed to be a highly specific inhibitor of p70S6K1. It is used in micromolar concentration range to dissect signaling pathways downstream of mTORC1 and to study the function of p70S6K1. Here we show that PF-4708671 induces AMP-activated protein kinase (AMPK) phosphorylation and activation in immortalized mouse embryonic fibroblasts (MEF) independently of p70S6K1, due to specific inhibition of mitochondrial respiratory chain Complex I.

Original languageEnglish
Article numbere0107364
JournalPLoS ONE
Volume9
Issue number9
DOIs
StatePublished - Sep 2014

Bibliographical note

Publisher Copyright:
© 2014 Vainer et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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