Phenytoin and transmitter release at the neuromuscular junction of the frog

Yoel Yaari; Jonathan H. Pincus; Zohar Argov

doi:10.1016/0006-8993(79)91074-6

Phenytoin and transmitter release at the neuromuscular junction of the frog

Yoel Yaari^*, Jonathan H. Pincus, Zohar Argov

^*Corresponding author for this work

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

The effects of phenytoin (diphenylhydantoin, DPH) on transmitter release were studied at the frog neuromuscular junction. It was found that in Ringer's solutions containing a normal concentration of Ca²⁺ ions, DPH (1-2 × 10^-4 M) depresses neurally evoked transmitter release, whereas in Ca²⁺-deficient Ringer's solutions it produces an increase in evoked release. Spontaneous transmitter liberation is augmented by DPH under all the above conditions. An abrupt disappearance of the evoked response occasionally occured with stimulation at 0.5 Hz, but a normal response could be elicited by a second stimulus delivered shortly after the first. At 100-200 Hz, DPH regularly induced a partial block in synaptic transmission. In 8 mM MgCl₂, this phenomenon appeared at 50 Hz and developed into a total neuromuscular blockade.

Original language	English
Pages (from-to)	479-487
Number of pages	9
Journal	Brain Research
Volume	160
Issue number	3
DOIs	https://doi.org/10.1016/0006-8993(79)91074-6
State	Published - 19 Jan 1979

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abstract = "The effects of phenytoin (diphenylhydantoin, DPH) on transmitter release were studied at the frog neuromuscular junction. It was found that in Ringer's solutions containing a normal concentration of Ca2+ ions, DPH (1-2 × 10-4 M) depresses neurally evoked transmitter release, whereas in Ca2+-deficient Ringer's solutions it produces an increase in evoked release. Spontaneous transmitter liberation is augmented by DPH under all the above conditions. An abrupt disappearance of the evoked response occasionally occured with stimulation at 0.5 Hz, but a normal response could be elicited by a second stimulus delivered shortly after the first. At 100-200 Hz, DPH regularly induced a partial block in synaptic transmission. In 8 mM MgCl2, this phenomenon appeared at 50 Hz and developed into a total neuromuscular blockade.",

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Phenytoin and transmitter release at the neuromuscular junction of the frog

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