Phosphonoacetic acid-resistant mutants of herpes simplex virus: effect of phosphonoacetic acid on virus replication and in vitro deoxyribonucleic acid synthesis in isolated nuclei

Y. Becker; Y. Asher; Y. Cohen; E. Weinberg-Zahlering; J. Shlomai

doi:10.1128/AAC.11.5.919

Phosphonoacetic acid-resistant mutants of herpes simplex virus: effect of phosphonoacetic acid on virus replication and in vitro deoxyribonucleic acid synthesis in isolated nuclei

Y. Becker
, Y. Asher
, Y. Cohen
, E. Weinberg-Zahlering
, J. Shlomai

Institute for Medical Research Israel-Canada (IMRIC)

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Phosphonoacetic acid (PAA) inhibits the replication of herpes simplex virus in BSC-1 cells and the in vitro synthesis of deoxyribonucleic acid (DNA) in isolated nuclei. Phosphonopropionic acid at a concentration of 100 μg/ml had no effect on herpes simplex virus replication. PAA-resistant mutants were obtained at a rate of 1 in 10⁴ plaque-forming units after 5-bromodeoxyuridine mutagenization of the virus. These mutants replicate in BSC-1 cells in the presence of 100 μg of PAA per ml and induce a PAA-resistant DNA polymerase that synthesizes DNA in vitro in the presence of PAA.

Original language	English
Pages (from-to)	919-922
Number of pages	4
Journal	Antimicrobial Agents and Chemotherapy
Volume	11
Issue number	5
DOIs	https://doi.org/10.1128/AAC.11.5.919
State	Published - 1977

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10.1128/AAC.11.5.919

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title = "Phosphonoacetic acid-resistant mutants of herpes simplex virus: effect of phosphonoacetic acid on virus replication and in vitro deoxyribonucleic acid synthesis in isolated nuclei",

abstract = "Phosphonoacetic acid (PAA) inhibits the replication of herpes simplex virus in BSC-1 cells and the in vitro synthesis of deoxyribonucleic acid (DNA) in isolated nuclei. Phosphonopropionic acid at a concentration of 100 μg/ml had no effect on herpes simplex virus replication. PAA-resistant mutants were obtained at a rate of 1 in 104 plaque-forming units after 5-bromodeoxyuridine mutagenization of the virus. These mutants replicate in BSC-1 cells in the presence of 100 μg of PAA per ml and induce a PAA-resistant DNA polymerase that synthesizes DNA in vitro in the presence of PAA.",

author = "Y. Becker and Y. Asher and Y. Cohen and E. Weinberg-Zahlering and J. Shlomai",

year = "1977",

doi = "10.1128/AAC.11.5.919",

language = "אנגלית",

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T2 - effect of phosphonoacetic acid on virus replication and in vitro deoxyribonucleic acid synthesis in isolated nuclei

AU - Becker, Y.

AU - Asher, Y.

AU - Cohen, Y.

AU - Weinberg-Zahlering, E.

AU - Shlomai, J.

PY - 1977

Y1 - 1977

N2 - Phosphonoacetic acid (PAA) inhibits the replication of herpes simplex virus in BSC-1 cells and the in vitro synthesis of deoxyribonucleic acid (DNA) in isolated nuclei. Phosphonopropionic acid at a concentration of 100 μg/ml had no effect on herpes simplex virus replication. PAA-resistant mutants were obtained at a rate of 1 in 104 plaque-forming units after 5-bromodeoxyuridine mutagenization of the virus. These mutants replicate in BSC-1 cells in the presence of 100 μg of PAA per ml and induce a PAA-resistant DNA polymerase that synthesizes DNA in vitro in the presence of PAA.

AB - Phosphonoacetic acid (PAA) inhibits the replication of herpes simplex virus in BSC-1 cells and the in vitro synthesis of deoxyribonucleic acid (DNA) in isolated nuclei. Phosphonopropionic acid at a concentration of 100 μg/ml had no effect on herpes simplex virus replication. PAA-resistant mutants were obtained at a rate of 1 in 104 plaque-forming units after 5-bromodeoxyuridine mutagenization of the virus. These mutants replicate in BSC-1 cells in the presence of 100 μg of PAA per ml and induce a PAA-resistant DNA polymerase that synthesizes DNA in vitro in the presence of PAA.

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AN - SCOPUS:0017685267

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JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 5

ER -

Phosphonoacetic acid-resistant mutants of herpes simplex virus: effect of phosphonoacetic acid on virus replication and in vitro deoxyribonucleic acid synthesis in isolated nuclei

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