TY - JOUR
T1 - Phosphorus bioavailability monitoring by a bioluminescent cyanobacterial sensor strain
AU - Gillor, Osnat
AU - Hadas, Ora
AU - Post, Anton F.
AU - Belkin, Shimshon
PY - 2002/1/1
Y1 - 2002/1/1
N2 - Phosphorus (P) is widely considered to be the main nutrient limiting the productivity of freshwater phytoplankton, but an assessment of its bioavailability in natural samples is highly complex. In an attempt to provide a novel tool for this purpose, the promoter of the alkaline phosphatase gene, phoA, from Synechococcus sp. PCC 7942 was fused to the luxAB luciferase genes of the bioluminescent bacterium Vibrio harveyi. The resulting construct was introduced into a neutral site on the Synechococcus sp. PCC 7942 genome to yield strain APL, which emitted light when inorganic P concentrations fell below 2.3 μM. Light emission of P-deprived cells decreased rapidly upon inorganic P readdition. The reporter was demonstrated to be a sensitive tool for monitoring the bioavailability of both inorganic and organic P sources. In water samples taken from a natural freshwater environment (Lake Kinneret, Israel), the luminescence measured correlated with total dissolved phosphate concentrations.
AB - Phosphorus (P) is widely considered to be the main nutrient limiting the productivity of freshwater phytoplankton, but an assessment of its bioavailability in natural samples is highly complex. In an attempt to provide a novel tool for this purpose, the promoter of the alkaline phosphatase gene, phoA, from Synechococcus sp. PCC 7942 was fused to the luxAB luciferase genes of the bioluminescent bacterium Vibrio harveyi. The resulting construct was introduced into a neutral site on the Synechococcus sp. PCC 7942 genome to yield strain APL, which emitted light when inorganic P concentrations fell below 2.3 μM. Light emission of P-deprived cells decreased rapidly upon inorganic P readdition. The reporter was demonstrated to be a sensitive tool for monitoring the bioavailability of both inorganic and organic P sources. In water samples taken from a natural freshwater environment (Lake Kinneret, Israel), the luminescence measured correlated with total dissolved phosphate concentrations.
KW - Alkaline phosphatase
KW - Bioluminescence
KW - PhoA
KW - Phosphorus bioavailability
KW - Phytoplankton
KW - Synechococcus sp. PCC 7942
UR - http://www.scopus.com/inward/record.url?scp=0036189251&partnerID=8YFLogxK
U2 - 10.1046/j.1529-8817.2002.01069.x
DO - 10.1046/j.1529-8817.2002.01069.x
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AN - SCOPUS:0036189251
SN - 0022-3646
VL - 38
SP - 107
EP - 115
JO - Journal of Phycology
JF - Journal of Phycology
IS - 1
ER -