Phosphorylated groucho delays differentiation in the follicle stem cell lineage by providing a molecular memory of EGFR signaling in the niche

Michael J. Johnston, Shaked Bar-Cohen, Ze’Ev Paroush, Todd G. Nystul*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

In the epithelial follicle stem cells (FSCs) of the Drosophila ovary, Epidermal Growth Factor Receptor (EGFR) signaling promotes selfrenewal, whereas Notch signaling promotes differentiation of the prefollicle cell (pFC) daughters. We have identified two proteins, Six4 and Groucho (Gro), that link the activity of these two pathways to regulate the earliest cell fate decision in the FSC lineage. Our data indicate that Six4 and Gro promote differentiation towards the polar cell fate by promoting Notch pathway activity. This activity of Gro is antagonized by EGFR signaling, which inhibits Gro-dependent repression via p-ERK mediated phosphorylation. We have found that the phosphorylated form of Gro persists in newly formed pFCs, which may delay differentiation and provide these cells with a temporary memory of the EGFR signal. Collectively, these findings demonstrate that phosphorylated Gro labels a transition state in the FSC lineage and describe the interplay between Notch and EGFR signaling that governs the differentiation processes during this period.

Original languageAmerican English
Pages (from-to)4631-4642
Number of pages12
JournalDevelopment (Cambridge)
Volume143
Issue number24
DOIs
StatePublished - 15 Dec 2016

Bibliographical note

Publisher Copyright:
© 2016. Published by The Company of Biologists Ltd.

Keywords

  • Drosophila
  • EGFR
  • Epithelial stem cell
  • Groucho
  • Ovary
  • Six4

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