Phosphorylation of Cdc5 regulates its accumulation

Kobi J. Simpson-Lavy, Michael Brandeis*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Background: Cdc5 (polo kinase/Plk1) is a highly conserved key regulator of the S. cerevisiae cell cycle from S-phase until cytokinesis. However, much of the regulatory mechanisms that govern Cdc5 remain to be determined. Cdc5 is phosphorylated on up to 10 sites during mitosis. In this study, we investigated the function of phosphorylation site T23, the only full consensus Cdk1 (Cdc28) phosphorylation site present.Findings: Cdc5 T23A introduces a degron that reduces its cellular amount to undetectable levels, which are nevertheless sufficient for normal cell proliferation. The degron acts in cis and is reversed by N-terminal GFP-tagging. Cdk1 kinase activity is required to maintain Cdc5 levels during G2. This, Cdk1 inhibited, Cdc5 degradation is APC/C Cdh1 independent and requires new protein synthesis. Cdc5 T23E is hyperactive, and reduces the levels of Cdc5 (in trans) and drastically reduces Clb2 levels.Conclusions: Phosphorylation of Cdc5 by Cdk1 is required to maintain Cdc5 levels during G2. However, phosphorylation of T23 (probably by Cdk1) caps Cdc5 and other CLB2 cluster protein accumulation, preventing potential protein toxicity, which may arise from their overexpression or from APC/C Cdh1 inactivation.

Original languageAmerican English
Article number23
JournalCell Division
Volume6
DOIs
StatePublished - 28 Dec 2011

Bibliographical note

Funding Information:
This research was supported by a grant from the United States-Israel Binational Science Foundation (BSF2007288), Jerusalem, Israel and by a Project Grant from the Israel Cancer Research Fund.

Keywords

  • Apc/c
  • Cdc28
  • Cdh1
  • Cdk1
  • Clb2
  • Mitosis
  • Phosphorylation
  • Polo
  • Swe1

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