TY - JOUR
T1 - Photoaffinity analog of herbicide inhibiting cellulose biosynthesis
T2 - Synthesis of [3H]‐2,6‐dichlorophenylazide
AU - Cooper, Geoffrey
AU - Delmer, Deborah
AU - Nitsche, Carmen
PY - 1987/7
Y1 - 1987/7
N2 - The compound 2,6‐dichlorobenzonitrile (dichlobenil, DCB) is known to be a reversible inhibitor of in vivo cellulose biosynthesis in higher plants and algae. We now report the synthesis of 2,6‐dichlorophenylazide (DCPA) with a non‐exchangeable tritium label of high specific activity. We have shown this compound to compete with DCB in binding to cotton fiber extracts, and to specifically label a low molecular weight protein in this extract on irradiation with UV light.
AB - The compound 2,6‐dichlorobenzonitrile (dichlobenil, DCB) is known to be a reversible inhibitor of in vivo cellulose biosynthesis in higher plants and algae. We now report the synthesis of 2,6‐dichlorophenylazide (DCPA) with a non‐exchangeable tritium label of high specific activity. We have shown this compound to compete with DCB in binding to cotton fiber extracts, and to specifically label a low molecular weight protein in this extract on irradiation with UV light.
KW - 2,6‐Dichlorobenzonitrile
KW - 2,6‐Dichlorophenylazide
KW - Biosynthesis
KW - Cellulose
KW - Photoaffinity
KW - Tritium
UR - http://www.scopus.com/inward/record.url?scp=0023196825&partnerID=8YFLogxK
U2 - 10.1002/jlcr.2580240705
DO - 10.1002/jlcr.2580240705
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AN - SCOPUS:0023196825
SN - 0362-4803
VL - 24
SP - 759
EP - 761
JO - Journal of Labelled Compounds and Radiopharmaceuticals
JF - Journal of Labelled Compounds and Radiopharmaceuticals
IS - 7
ER -