Photobiomodulation and estrogen stabilize mitochondrial membrane potential in angiotensin–II challenged porcine aortic smooth muscle cells

Lilach Gavish*, Dan Gilon, Ronen Beeri, Ayelet Zuckerman, Dean Nachman, S. David Gertz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Rupture of Abdominal aortic aneurysm (AAA) is among the 15 leading causes of death after age 65. Using high frequency ultrasound, we showed that photobiomodulation (PBM) prevents formation and progression of AAA in the angiotensin-II (Ang-II)-infused, apolipoprotein-e-deficient mouse model. In the current study we report that while challenge of porcine aortic Smooth Muscle Cells (SMCs) with Ang-II (1 μM) resulted in a marked decay in mitochondrial membrane potential (MitMP) vs non-challenged cells, treatment with PBM (continuous diode laser, 780 nm, 6.7 mW/cm2, 5 minutes, 2 J/cm2) or pre-incubation with estrogen (50 nM, 1 hour) significantly attenuated this deterioration in MitMP. We also report that PBM and estrogen markedly affected porcine aortic SMC contraction and modified mitochondrial dispersion reflecting important influence on SMC function. These studies provide strong evidence of the important underlying role of mitochondria in the preventive effect of PBM on formation and progression of AAA and its reduced incidence and delayed onset in women.

Original languageAmerican English
Article numbere202000329
JournalJournal of Biophotonics
Volume14
Issue number1
DOIs
StatePublished - Jan 2021

Bibliographical note

Funding Information:
The Alexander Grass Family Research Fund; The Bruce and Baila Waldholtz Research Fund; The Rosetrees Trust Fund of the United Kingdom, Grant/Award Number: M140‐F2; The Saul and Joyce Brandman Fund for Cardiovascular Research; The Stuart Roden Family (Landsdowne Partners) Research Fund Funding information −/−

Funding Information:
S. D. G. is the Brandman Foundation Professor of Cardiac and Pulmonary Diseases of The Faculty of Medicine, The Hebrew University of Jerusalem. The authors gratefully acknowledge the expert technical assistance of Dr. Mark Tarshish, Dr. Yael Feinstein‐Rotkopf, and Dr. Zakhariya Manevitch from the Light Microscopy and Image Analysis Laboratory of The Core Research Facility (CRF), Faculty of Medicine. These studies were supported in part by The Saul and Joyce Brandman Fund for Cardiovascular Research; The Rosetrees Trust Fund of the United Kingdom (M140‐F2); The Stuart Roden Family (Landsdowne Partners) Research Fund, London, UK; The Alexander Grass Family Research Fund, and The Bruce and Baila Waldholtz Research Fund, The Hebrew University of Jerusalem.

Publisher Copyright:
© 2020 Wiley-VCH GmbH

Keywords

  • abdominal aortic aneurysm
  • estradiol
  • low-level laser
  • mitochondrial dysfunction
  • photobiomodulation

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