TY - JOUR
T1 - Photoelectrochemical biosensors without external irradiation
T2 - Probing enzyme activities and DNA sensing using Hemin/G-quadruplex-stimulated chemiluminescence resonance energy transfer (CRET) generation of photocurrents
AU - Golub, Eyal
AU - Niazov, Angelica
AU - Freeman, Ronit
AU - Zatsepin, Maria
AU - Willner, Itamar
PY - 2012/6/28
Y1 - 2012/6/28
N2 - A hemin/G-quadruplex nanostructure that is immobilized on CdS quantum dots (QDs) associated with an electrode leads, in the presence of luminol, H 2O 2, and triethanolamine as an electron donor, to the generation of photocurrents with no external irradiation of the QDs. The hemin/G-quadruplex-catalyzed generation of chemiluminescence leads to the chemiluminescence resonance energy transfer (CRET) to the QDs, resulting in the photoexcitation of the QDs and the generation of electron-hole pairs. The transfer of the conduction-band electrons to the electrode, and the concomitant scavenging of the valence-band holes by the triethanolamine electron donor result in the generation of photocurrents. The CRET-stimulated generation of photocurrents is applied to sense DNA by the labeling of the probe-analyte complex with a hemin/G-quadruple, and is also implemented to follow the activity of glucose oxidase and to sense glucose, by the labeling of the enzyme with the hemin/G-quadruplex catalyst.
AB - A hemin/G-quadruplex nanostructure that is immobilized on CdS quantum dots (QDs) associated with an electrode leads, in the presence of luminol, H 2O 2, and triethanolamine as an electron donor, to the generation of photocurrents with no external irradiation of the QDs. The hemin/G-quadruplex-catalyzed generation of chemiluminescence leads to the chemiluminescence resonance energy transfer (CRET) to the QDs, resulting in the photoexcitation of the QDs and the generation of electron-hole pairs. The transfer of the conduction-band electrons to the electrode, and the concomitant scavenging of the valence-band holes by the triethanolamine electron donor result in the generation of photocurrents. The CRET-stimulated generation of photocurrents is applied to sense DNA by the labeling of the probe-analyte complex with a hemin/G-quadruple, and is also implemented to follow the activity of glucose oxidase and to sense glucose, by the labeling of the enzyme with the hemin/G-quadruplex catalyst.
UR - http://www.scopus.com/inward/record.url?scp=84863098880&partnerID=8YFLogxK
U2 - 10.1021/jp303741x
DO - 10.1021/jp303741x
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AN - SCOPUS:84863098880
SN - 1932-7447
VL - 116
SP - 13827
EP - 13834
JO - Journal of Physical Chemistry C
JF - Journal of Physical Chemistry C
IS - 25
ER -