TY - JOUR
T1 - Photoinactivation and identification of the biogenic amine transporter in chromaffin granules from bovine adrenal medulla
AU - Gabizon, R.
AU - Yetinson, T.
AU - Schuldiner, S.
PY - 1982
Y1 - 1982
N2 - We have synthesized a new derivative of 5-hydroxytryptamine, a substrate of the biogenic amine transporter in bovine chromaffin granules. This new compound, 4-azido, 3-nitrophenyl-azo-(5-hyroxytryptamine), is a competitive, reversible, and specific inhibitor of the biogenic amine transporter in the dark (K(i) = 2 μM). Upon illumination, the inactivation of the transporter becomes irreversible and follows an apparent first order rate kinetics (K(INAC) = 4 μM). The transporter can be protected if other substrates are present during illumination. When [3H]4-azido,3-nitrophenyl-azo-(5-hydroxytryptamine) is used, preferential labeling of one polypeptide (M(r) ~ 45,000) is observed in the light, provided no other substrates of the transporter are present during illumination. We have tentatively identified this polypeptide as the transporter itself or one of its subunits.
AB - We have synthesized a new derivative of 5-hydroxytryptamine, a substrate of the biogenic amine transporter in bovine chromaffin granules. This new compound, 4-azido, 3-nitrophenyl-azo-(5-hyroxytryptamine), is a competitive, reversible, and specific inhibitor of the biogenic amine transporter in the dark (K(i) = 2 μM). Upon illumination, the inactivation of the transporter becomes irreversible and follows an apparent first order rate kinetics (K(INAC) = 4 μM). The transporter can be protected if other substrates are present during illumination. When [3H]4-azido,3-nitrophenyl-azo-(5-hydroxytryptamine) is used, preferential labeling of one polypeptide (M(r) ~ 45,000) is observed in the light, provided no other substrates of the transporter are present during illumination. We have tentatively identified this polypeptide as the transporter itself or one of its subunits.
UR - http://www.scopus.com/inward/record.url?scp=0020448945&partnerID=8YFLogxK
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 7174689
AN - SCOPUS:0020448945
SN - 0021-9258
VL - 257
SP - 15145
EP - 15150
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 24
ER -