Photosystem I reaction centers from maize bundle‐sheath and mesophyll chloroplasts lack subunit III

Phtosystem I reaction centers were isolated from mesophyll and bundle‐sheath chloroplasts of the C₄ maize plant. Both preparations were found to be free of chlorophyll b and to have the same spectral properties and chlorophyll/P700 ratio as photosystem I reaction centers isolated from C₃ plants. Photosystem I reaction centers from both mesophyll and bundle sheath were found to consist of six subunits with apparent molecular masses of about 70 kDa, 20 kDa, 17 kDa, 16 kDa, 10 kDa and 8 kDa, corresponding to photosystem I reaction center subunits I, II, IV, V, VI and VII of spinach, as tested by their immunological cross‐reactivity with antibody raised against the respective spinach subunits. No cross‐reactivity was found with antibodies raised against subunit III of spinach, either in whole thylakoids or purified reaction centers of both bundle‐sheath and mesophyll chloroplasts. It is concluded that photosystem I reaction centers of bundle‐sheath and mesophyll thylakoids of maize are identical and lack the polypeptide corresponding to subunit III present in all C₃ plants so far tested.