Physical mapping of the ribosomal RNA genes of Mycoplasma capricolum

Gad Glaser*, Dorit Amikam, Shmuel Razin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Physical mapping of the rRNA genes of Mycoplasma capricolum was done by digestion of the mycoplasmal DNA with EcoRI, PstI and Bg1II and hybridization with nick-translated probes consisting of defined portions of the rrnB ribosomal RNA operon of Escherichia coli. The results indicate that the rRNA genes in the chromosome of M. capricolum are arranged in two clusters, each organized in the order 5′-16S-23S-5S-3′, resembling the ordar of the genes in the rrnB operon, with no large spacer regions separating the genes in each cluster.

Original languageEnglish
Pages (from-to)2421-2426
Number of pages6
JournalNucleic Acids Research
Volume12
Issue number5
DOIs
StatePublished - 12 Mar 1984

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