Physical Responses of Bacterial Chemoreceptors

Ady Vaknin, Howard C. Berg*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

78 Scopus citations

Abstract

Chemoreceptors of the bacterium Escherichia coli are thought to form trimers of homodimers that undergo conformational changes upon ligand binding and thereby signal a cytoplasmic kinase. We monitored the physical responses of trimers in living cells lacking other chemotaxis proteins by fluorescently tagging receptors and measuring changes in fluorescence anisotropy. These changes were traced to changes in energy transfer between fluorophores on different dimers of a trimer: attractants move these fluorophores farther apart, and repellents move them closer together. These measurements allowed us to define the responses of bare receptor oligomers to ligand binding and compare them to the corresponding response in kinase activity. Receptor responses could be fit by a simple "two-state" model in which receptor dimers are in either active or inactive conformations, from which energy bias and dissociation constants could be estimated. Comparison with responses in kinase-activity indicated that higher-order interactions are dominant in receptor clusters.

Original languageAmerican English
Pages (from-to)1416-1423
Number of pages8
JournalJournal of Molecular Biology
Volume366
Issue number5
DOIs
StatePublished - 9 Mar 2007
Externally publishedYes

Bibliographical note

Funding Information:
We thank V. Sourjik and J.S. Parkinson for strains and plasmids, T.S. Shimizu for helpful conversations, and J.S. Parkinson and J.J. Falke for their comments on the manuscript. This work was supported by National Institutes of Health grant AI016478.

Keywords

  • chemotaxis
  • fluorescence polarization
  • fluorescence resonance energy transfer
  • membrane proteins
  • receptors

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