Physically interacting beta-delta pairs in the regenerating pancreas revealed by single-cell sequencing

Eran Yanowski, Nancy S. Yacovzada, Eyal David, Amir Giladi, Diego Jaitin, Lydia Farack, Adi Egozi, Danny Ben-Zvi, Shalev Itzkovitz, Ido Amit, Eran Hornstein*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Objectives: Until recently, communication between neighboring cells in islets of Langerhans was overlooked by genomic technologies, which require rigorous tissue dissociation into single cells. Methods: We utilize sorting of physically interacting cells (PICs) with single-cell RNA-sequencing to systematically map cellular interactions in the endocrine pancreas after pancreatectomy. Results: The pancreas cellular landscape features pancreatectomy associated heterogeneity of beta-cells, including an interaction-specific program between paired beta and delta-cells. Conclusions: Our analysis suggests that the particular cluster of beta-cells that pairs with delta-cells benefits from stress protection, implying that the interaction between beta- and delta-cells might safeguard against pancreatectomy associated challenges. The work encourages testing the potential relevance of physically-interacting beta-delta-cells also in diabetes mellitus.

Original languageAmerican English
Article number101467
JournalMolecular Metabolism
Volume60
DOIs
StatePublished - Jun 2022

Bibliographical note

Publisher Copyright:
© 2022 The Authors

Keywords

  • Endocrine pancreas
  • Islet of Langerhans
  • Pancreas regeneration
  • Pancreatectomy
  • Single-cell RNA-sequencing
  • Single-cell transcriptome sequencing
  • beta-delta cell pair
  • scRNA-seq

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