Physiological age structure and Leishmania spp. detection in phlebotomus (Larroussius) orientalis (Parrot, 1936) (Diptera: Psychodidae) at an endemic focus of visceral leishmaniasis in northern Ethiopia

Araya Gebresilassie*, Ibrahim Abbasi, Oscar David Kirstein, Essayas Aklilu, Solomon Yared, Habte Tekie, Meshesha Balkew, Alon Warburg, Asrat Hailu, Teshome Gebre-Michael

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Visceral leishmaniasis (VL) caused by Leishmania donovani is endemic in northern Ethiopia, where P. orientalis is the most important presumed vector. This study was designed to determine the physiological age structure and the occurrence of Leishmania infection in the vector of VL in Tahtay Adiyabo district, northern Ethiopia. Sand flies were collected using CDC light traps from peridomestic and agricultural fields between May 2011 and April 2012 and P. orientalis females were dissected for age determination and detection of Leishmania promastigotes. Sand flies were also analyzed for L. donovani detection using molecular methods. Of 1,282 P. orientalis examined for abdominal stages and age characterization, 66.2%, 28.2%, 4.1%, and 1.6% were unfed, freshly fed, half-gravid, and gravid. Parous rate in unfed females was 34.1% and 35.4% in peridomestic and agricultural fields, respectively. Out of 921 P. orientalis females dissected, one specimen (0.1%) was found naturally infected with promastigotes. Five pools (25 females) of unfed P. orientalis were also found with DNA of Leishmania spp. In particular, a single P. orientalis was positive for L. donovani (0.5%). Based on this and other evidences (abundance, human blood feeding, and xenodiagnostic studies), P. orientalis is the principal vector of VL in this endemic focus.

Original languageAmerican English
Article number710528
JournalJournal of Tropical Medicine
Volume2015
DOIs
StatePublished - 2015

Bibliographical note

Publisher Copyright:
© 2015 Araya Gebresilassie et al.

Fingerprint

Dive into the research topics of 'Physiological age structure and Leishmania spp. detection in phlebotomus (Larroussius) orientalis (Parrot, 1936) (Diptera: Psychodidae) at an endemic focus of visceral leishmaniasis in northern Ethiopia'. Together they form a unique fingerprint.

Cite this