Pitfalls and artifacts in measuring absorption spectra and kinetics: The effect of stray light in the UV and red regions

Gideon Czapski, Yair Ozeri, Sara Goldstein

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Effects of stray light on absorption spectrum and kinetics are discussed. The extent of the stray light depends on the light source, monochromator, wavelength set by the instrument and the absorption of the sample at this wavelength. Effects of the stray light on the shape of the spectrum and the extinction coefficients are shown. Methods for determining the existence and extent of stray light are suggested and are especially relevant for studies using pulse radiolysis, flash photolysis, and stopped-flow techniques. The literature examples for artifacts due to stray light are presented for kinetics and absorption spectra.

Original languageEnglish
Pages (from-to)229-234
Number of pages6
JournalRadiation Physics and Chemistry
Volume72
Issue number2-3
DOIs
StatePublished - Feb 2005

Keywords

  • Absorption spectrum
  • Artifact
  • Kinetics
  • Stray light

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