Plant Regeneration from Callus Cultures of Allium trifoliatum subsp. hirsutum and Assessment of Genetic Stability by Isozyme Polymorphism

A. Viterbo, H. D. Rabinowitch*, A. Altman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Plant regeneration from callus cultures of Allium trifoliatum subsp. hirsutum fertile accession F‐370, was studied as a means for clonal multiplication and germplasm storage of Allium spp. Callus was induced on in votro‐cultured basal leaf explants. Best proliferation was obtained on modified BDS medium supplemented with (mg/1): 0.75 picloram, 2.0 benzyl adenine, and 900 casein hydrolysate. Shoot and root organogenesis were obtained in 3 to 5 month old subcultured calli, on BDS or MS medium supplemented with (mg/1): either 0.03 picloram or no auxin, 2 BA or 2‐isopentenyladenine, and 900 casein hydrolysate. Direct bulb formation, without shoot elongation, occurred on BDS medium with 10 mg/1 IBA. Under these conditions, callus formation and organogenesis were not obtained with A. trifoliatum subsp. hirsutum var. sterile, a male‐sterile genotype. Most regenerants were phenotypically normal, but some abnormal shoots were also observed, i.e. shoots with vitrified or extremely broad leaves. Isozyme polymorphism analysis of seven proteins in the latter regenerants, and in several callus cultures, revealed significant deviation from the original pattern in esterase, 6‐phosphogluconate dehydrogenase and superoxide dismutase. No such deviations were detected in normal regenerated plants.

Original languageEnglish
Pages (from-to)265-273
Number of pages9
JournalPlant Breeding
Volume108
Issue number4
DOIs
StatePublished - May 1992

Keywords

  • Allium trifoliatum
  • callus
  • germ‐plasm
  • isozyme polymorphism
  • somaclonal variation

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