TY - JOUR
T1 - Plant Regeneration from Callus Cultures of Allium trifoliatum subsp. hirsutum and Assessment of Genetic Stability by Isozyme Polymorphism
AU - Viterbo, A.
AU - Rabinowitch, H. D.
AU - Altman, A.
PY - 1992/5
Y1 - 1992/5
N2 - Plant regeneration from callus cultures of Allium trifoliatum subsp. hirsutum fertile accession F‐370, was studied as a means for clonal multiplication and germplasm storage of Allium spp. Callus was induced on in votro‐cultured basal leaf explants. Best proliferation was obtained on modified BDS medium supplemented with (mg/1): 0.75 picloram, 2.0 benzyl adenine, and 900 casein hydrolysate. Shoot and root organogenesis were obtained in 3 to 5 month old subcultured calli, on BDS or MS medium supplemented with (mg/1): either 0.03 picloram or no auxin, 2 BA or 2‐isopentenyladenine, and 900 casein hydrolysate. Direct bulb formation, without shoot elongation, occurred on BDS medium with 10 mg/1 IBA. Under these conditions, callus formation and organogenesis were not obtained with A. trifoliatum subsp. hirsutum var. sterile, a male‐sterile genotype. Most regenerants were phenotypically normal, but some abnormal shoots were also observed, i.e. shoots with vitrified or extremely broad leaves. Isozyme polymorphism analysis of seven proteins in the latter regenerants, and in several callus cultures, revealed significant deviation from the original pattern in esterase, 6‐phosphogluconate dehydrogenase and superoxide dismutase. No such deviations were detected in normal regenerated plants.
AB - Plant regeneration from callus cultures of Allium trifoliatum subsp. hirsutum fertile accession F‐370, was studied as a means for clonal multiplication and germplasm storage of Allium spp. Callus was induced on in votro‐cultured basal leaf explants. Best proliferation was obtained on modified BDS medium supplemented with (mg/1): 0.75 picloram, 2.0 benzyl adenine, and 900 casein hydrolysate. Shoot and root organogenesis were obtained in 3 to 5 month old subcultured calli, on BDS or MS medium supplemented with (mg/1): either 0.03 picloram or no auxin, 2 BA or 2‐isopentenyladenine, and 900 casein hydrolysate. Direct bulb formation, without shoot elongation, occurred on BDS medium with 10 mg/1 IBA. Under these conditions, callus formation and organogenesis were not obtained with A. trifoliatum subsp. hirsutum var. sterile, a male‐sterile genotype. Most regenerants were phenotypically normal, but some abnormal shoots were also observed, i.e. shoots with vitrified or extremely broad leaves. Isozyme polymorphism analysis of seven proteins in the latter regenerants, and in several callus cultures, revealed significant deviation from the original pattern in esterase, 6‐phosphogluconate dehydrogenase and superoxide dismutase. No such deviations were detected in normal regenerated plants.
KW - Allium trifoliatum
KW - callus
KW - germ‐plasm
KW - isozyme polymorphism
KW - somaclonal variation
UR - http://www.scopus.com/inward/record.url?scp=84989036325&partnerID=8YFLogxK
U2 - 10.1111/j.1439-0523.1992.tb00130.x
DO - 10.1111/j.1439-0523.1992.tb00130.x
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:84989036325
SN - 0179-9541
VL - 108
SP - 265
EP - 273
JO - Plant Breeding
JF - Plant Breeding
IS - 4
ER -