Plasma Factor in Red Blood Cells Adhesion to Endothelial Cells: Humans and Rats

Avraham Schlager, Gideon Zamir, Gregory Barshtein*, Saul Yedgar, Dan Arbell

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Erythrocyte adhesion to the vascular endothelium is one of the key determinants of microcirculatory blood flow. Adhesion is a complex process determined by the intricate interaction among red blood cells (RBC), plasma factors, and the vascular endothelium. Rats are commonly used as disease models to investigate the pathophysiology of various hematological disease processes occurring in humans and their response to prospective treatments. The aim of our study was to characterize the adhesion of RBC in adult blood from rat and human subjects, in order to test the validity of rat models for adhesion-related disease processes. We demonstrated that adhesion of RBC from rats (rRBC), to endothelial cells (EC) in plasma-free buffer, is stronger than from human subjects (hRBC). In addition, plasma proteins induced elevation of hRBC (eightfold) but depression of rRBC (threefold) adhesion to EC. It is thus suggested to be aware of the difference in RBC/EC interaction for human and rat subjects, when studying models of blood flow.

Original languageAmerican English
Pages (from-to)157-161
Number of pages5
JournalCell Biochemistry and Biophysics
Volume58
Issue number3
DOIs
StatePublished - 2010

Bibliographical note

Funding Information:
Acknowledgments This work was supported by grants from the National Blood Foundation (to S. Yedgar and G. Barshtein), the Israel Science Foundation No. 558/03 (to S. Yedgar and G. Barshtein), and the Walter and Greta Stiel Chair for Heart Studies (to S. Yedgar). The authors thank Mrs. O. Fredman for his technical assistance.

Keywords

  • Animal model
  • Blood plasma
  • Erythrocyte adhesion

Fingerprint

Dive into the research topics of 'Plasma Factor in Red Blood Cells Adhesion to Endothelial Cells: Humans and Rats'. Together they form a unique fingerprint.

Cite this