Plasma membrane aquaporins in the motor cells of Samanea saman: Diurnal and circadian regulation

Menachem Moshelion; Dirk Becker; Alexander Biela; Norbert Uehlein; Rainer Hedrich; Beate Otto; Hadas Levi; Nava Moran; Ralf Kaldenhoff

doi:10.1105/tpc.010351

Plasma membrane aquaporins in the motor cells of Samanea saman: Diurnal and circadian regulation

Menachem Moshelion, Dirk Becker, Alexander Biela, Norbert Uehlein, Rainer Hedrich, Beate Otto, Hadas Levi, Nava Moran^*, Ralf Kaldenhoff

^*Corresponding author for this work

Institute of Plant Sciences and Genetics in Agriculture

Research output: Contribution to journal › Article › peer-review

191 Scopus citations

Abstract

Leaf-moving organs, remarkable for the rhythmic volume changes of their motor cells, served as a model system in which to study the regulation of membrane water fluxes. Two plasma membrane intrinsic protein homolog genes, SsAQP1 and SsAQP2, were cloned from these organs and characterized as aquaporins in Xenopus laevis oocytes. Osmotic water permeability (P_f) was 10 times higher in SsAQP2-expressing oocytes than in SsAQP1-expressing oocytes. SsAQP1 was found to be glycerol permeable, and SsAQP2 was inhibited by 0.5 mM HgCl2 and by 1 mM phloretin. The aquaporin mRNA levels differed in their spatial distribution in the leaf and were regulated diurnally in phase with leaflet movements. Additionally, SsAQP2 transcription was under circadian control. The P_f of motor cell protoplasts was regulated diurnally as well: the morning and/or evening P_f increases were inhibited by 50 μM HgCl₂, by 2 mM cycloheximide, and by 250 μM phloretin to the noon P_f level. Our results link SsAQP2 to the physiological function of rhythmic cell volume changes.

Original language	English
Pages (from-to)	727-739
Number of pages	13
Journal	Plant Cell
Volume	14
Issue number	3
DOIs	https://doi.org/10.1105/tpc.010351
State	Published - 2002

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title = "Plasma membrane aquaporins in the motor cells of Samanea saman: Diurnal and circadian regulation",

abstract = "Leaf-moving organs, remarkable for the rhythmic volume changes of their motor cells, served as a model system in which to study the regulation of membrane water fluxes. Two plasma membrane intrinsic protein homolog genes, SsAQP1 and SsAQP2, were cloned from these organs and characterized as aquaporins in Xenopus laevis oocytes. Osmotic water permeability (Pf) was 10 times higher in SsAQP2-expressing oocytes than in SsAQP1-expressing oocytes. SsAQP1 was found to be glycerol permeable, and SsAQP2 was inhibited by 0.5 mM HgCl2 and by 1 mM phloretin. The aquaporin mRNA levels differed in their spatial distribution in the leaf and were regulated diurnally in phase with leaflet movements. Additionally, SsAQP2 transcription was under circadian control. The Pf of motor cell protoplasts was regulated diurnally as well: the morning and/or evening Pf increases were inhibited by 50 μM HgCl2, by 2 mM cycloheximide, and by 250 μM phloretin to the noon Pf level. Our results link SsAQP2 to the physiological function of rhythmic cell volume changes.",

author = "Menachem Moshelion and Dirk Becker and Alexander Biela and Norbert Uehlein and Rainer Hedrich and Beate Otto and Hadas Levi and Nava Moran and Ralf Kaldenhoff",

year = "2002",

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AB - Leaf-moving organs, remarkable for the rhythmic volume changes of their motor cells, served as a model system in which to study the regulation of membrane water fluxes. Two plasma membrane intrinsic protein homolog genes, SsAQP1 and SsAQP2, were cloned from these organs and characterized as aquaporins in Xenopus laevis oocytes. Osmotic water permeability (Pf) was 10 times higher in SsAQP2-expressing oocytes than in SsAQP1-expressing oocytes. SsAQP1 was found to be glycerol permeable, and SsAQP2 was inhibited by 0.5 mM HgCl2 and by 1 mM phloretin. The aquaporin mRNA levels differed in their spatial distribution in the leaf and were regulated diurnally in phase with leaflet movements. Additionally, SsAQP2 transcription was under circadian control. The Pf of motor cell protoplasts was regulated diurnally as well: the morning and/or evening Pf increases were inhibited by 50 μM HgCl2, by 2 mM cycloheximide, and by 250 μM phloretin to the noon Pf level. Our results link SsAQP2 to the physiological function of rhythmic cell volume changes.

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Plasma membrane aquaporins in the motor cells of Samanea saman: Diurnal and circadian regulation

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