Plasmodium berghei: I. Immunochemical properties of fractions of a soluble extract

Joseph Hamburger*, Avivah Zuckerman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Soluble extracts of Plasmodium berghei were separated into 12 fractions following preparative disc electrophoresis in polyacrylamide gel. One or two protein bands were detected in each fraction by analytical disc electrophoresis. Similarly, one or two precipitinogens were generally detected in each of Fractions 1 through 11 by immunoelectrophoresis and by double immunodiffusion in agar gel, while the unfractionated extract contained 10 precipitinogens. Antisera produced in rabbits against each fraction each contained two or three (sometimes five) antiplasmodial precipitins demonstrable by immunoelectrophoresis. Serial fractions obtained in separate runs were closely similar to each other, although some degree of overlapping sometimes occurred between neighboring fractions. Glycoproteins were detected in all the fractions, but chiefly in Fractions 4 and 12. The bulk of the RNA in the extract was located in Fraction 4, while hemoglobin was usually confined to Fraction 6. The molecular weights of the soluble components of P. berghei range between 8000 and 130,000.

Original languageEnglish
Pages (from-to)460-478
Number of pages19
JournalExperimental Parasitology
Volume39
Issue number3
DOIs
StatePublished - Jun 1976

Keywords

  • Double immunodiffusion
  • Electrophoresis, disc (analytical and preparative)
  • Electrophoretic elution of fractions
  • Glycoproteins
  • Hemoglobin
  • Immunoelectrophoresis
  • Mice
  • Molecular weights
  • Mouse malaria
  • Plasmodium berghei
  • Precipitinogens
  • Rabbits
  • RNA
  • Soluble extract

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