Plasmodium falciparum: Expression of the adenine phosphoribosyltransferase gene in mouse L cells

Yaakov Pollack*, Ruth Shemer, Shulamit Metzger, Dan T. Spira, Jacob Golenser

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Genomic libraries of Plasmodium falciparum were constructed in the pBR322 plasmid. Using the DNA-mediated gene transfer technique, the genomic libraries were introduced into tissue-cultured mouse cells lacking the enzyme adenine phosphoribosyltransferase. Following selection for the adenine phosphoribosyltransferse phenotype, several colonies were isolated. All clones were shown to possess adenine phosphoribosyltransferase activity and pBR322 sequences. In addition, the Km value of adenine phosphoribosyltransferase (for adenine) from a transformant was found to be identical to that from P. falciparum. These results indicate that the adenine phosphoribosyltransferase gene of P. falciparum was successfully cloned and expressed in a mammalian system.

Original languageAmerican English
Pages (from-to)270-275
Number of pages6
JournalExperimental Parasitology
Issue number3
StatePublished - Dec 1985

Bibliographical note

Funding Information:
This study was supported by the Lester Aronberg Foundation.


  • Adenine phosphoribosyltransferse (arpt) expression
  • DNA
  • Gene transfer
  • Malaria, human
  • Mouse L cells
  • Plasmodium falciparum
  • Protozoa, parasitic


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