TY - JOUR
T1 - Plasmodium falciparum
T2 - Synchronization of cultures with dl-α-difluoromethylornithine, an inhibitor of polyamine biosynthesis
AU - Assaraf, Yehuda G.
AU - Golenser, Jacob
AU - Spira, Dan T.
AU - Bachrach, Uriel
PY - 1986/4
Y1 - 1986/4
N2 - dl-α-Difluoromethylornithine, an inhibitor of polyamine biosynthesis, was tested for its ability to synchronize Plasmodium falciparum. Asynchronous cultures were pretreated with sorbitol and incubated for 28-30 hr. Then, when cultures consisted of mainly schizont stage parasites, dl-α-difluoromethylornithine was added to the growth medium for another 38-47 hr of incubation. Putrescine was added to parasites arrested at the early trophozoite stage. This resulted in a synchronous resumption of growth. After 19 hr, 83% of parasites were at the schizont stage. After 30 hr, more than 98% of the parasites were in the ring form stage. Furthermore, the transformation of early trophozoites to schizonts occurred within 3 hr, with a slight reduction in parasitemia. Synchrony was maintained for 4-5 biological cycles as confirmed also by flow fluorimetry. It appears that this new approach to synchronize P. falciparum cultures is simple, reproducible, and effective.
AB - dl-α-Difluoromethylornithine, an inhibitor of polyamine biosynthesis, was tested for its ability to synchronize Plasmodium falciparum. Asynchronous cultures were pretreated with sorbitol and incubated for 28-30 hr. Then, when cultures consisted of mainly schizont stage parasites, dl-α-difluoromethylornithine was added to the growth medium for another 38-47 hr of incubation. Putrescine was added to parasites arrested at the early trophozoite stage. This resulted in a synchronous resumption of growth. After 19 hr, 83% of parasites were at the schizont stage. After 30 hr, more than 98% of the parasites were in the ring form stage. Furthermore, the transformation of early trophozoites to schizonts occurred within 3 hr, with a slight reduction in parasitemia. Synchrony was maintained for 4-5 biological cycles as confirmed also by flow fluorimetry. It appears that this new approach to synchronize P. falciparum cultures is simple, reproducible, and effective.
KW - Malaria, human
KW - Plasmodium falciparum
KW - Polyamines
KW - Protozoa, parasitic
UR - http://www.scopus.com/inward/record.url?scp=0022506979&partnerID=8YFLogxK
U2 - 10.1016/0014-4894(86)90156-6
DO - 10.1016/0014-4894(86)90156-6
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C2 - 3082665
AN - SCOPUS:0022506979
SN - 0014-4894
VL - 61
SP - 229
EP - 235
JO - Experimental Parasitology
JF - Experimental Parasitology
IS - 2
ER -