Plasmodium gallinaceum: Antibodies to circumsporozoite protein prevent sporozoites from invading the salivary glands of Aedes aegypti

Alon Warburg*, Musa Touray, Antoniana U. Krettli, Louis H. Miller

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

38 Scopus citations


A Circumsporozoite protein-specific monoclonal antibody (N2H6D5) was injected into malaria-infected mosquitoes to determine its effect on the sporogonic cycle. After injection of antibody into mosquitoes (100 ng each), positive immunofluorescence (measured on air-dried sporozoites) reactions in hemolymph extracts were observed at a dilution of 1:1000. At 72 hr postinjection the levels dropped to 1:10. Sporozoites coinjected with antibody did not invade the salivary glands. In naturally infected mosquitoes, sporozoites were released over a period of 3 to 4 days. Therefore, mosquitoes were injected twice. The first injection was a day before the beginning of sporozoite release and the second, 2 days later. Sporozoite invasion of the salivary glands was assessed 3 days after the second injection, by microscopic examination of dissected glands. At this stage, all oocysts had completed maturation and released the sporozoites. Salivary gland infections were totally prevented in mosquitoes given two injections of 100 ng N2H6D5. Hence, sustained presence of anti-circumsporozoite antibodies in the hemolymph can render female Aedes aegypti refractory to Plasmodium gallinaceum.

Original languageAmerican English
Pages (from-to)303-307
Number of pages5
JournalExperimental Parasitology
Issue number3
StatePublished - Nov 1992
Externally publishedYes

Bibliographical note

Funding Information:
*Laboratory of Malaria Research, National Institute of Allergy 6r Infectious Diseases, National Institutes of Health, Building 4, Room 126, Bethesda, Maryland 20892, U.S.A.; and Kentro de Pesquisas Rene Rachou, Avenida August0 de Lima 1715, Caixa Postal 1743, 30190-Belo Horizonte-MG, Brazil


  • Aedes aegypti
  • Circumsporozoite proteins
  • Malaria
  • Monoclonal antibodies to Circumsporozoite proteins
  • Mosquito salivary glands
  • Plasmodium gallinaceum
  • Sporozoite infectivity


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