TY - JOUR
T1 - Platform for Active Vaccine Formulation Using a Two-Mode Enhancement Mechanism of Epitope Presentation by Pickering Emulsion
AU - Mechrez, Guy
AU - Mani, Karthik Ananth
AU - Saha, Abhijit
AU - Lachman, Oded
AU - Luria, Neta
AU - Molad, Ori
AU - Kotliarevski, Liliya
AU - Zelinger, Einat
AU - Smith, Elisheva
AU - Yaakov, Noga
AU - Stone, Dalia Shabashov
AU - Reches, Meital
AU - Dombrovsky, Aviv
N1 - Publisher Copyright:
© 2022 American Chemical Society.
PY - 2022/8/15
Y1 - 2022/8/15
N2 - The efficiency of epitope-based vaccination (subunit vaccines) is tightly correlated with heterogeneity and the high density of epitope presentation, which maximizes the potential antigenic determinants. Here, we developed a two-mode platform for intensifying the epitope presentation of subunit vaccines. The two-mode epitope presentation enhancement includes a covalent attachment of high concentrations of SARS-CoV-2-S1 peptide epitope to the surface of virus-like-particles (VLPs) and the subsequent assembly of VLP/epitope conjugates on the oil droplet surface at an oil/water interface of an emulsion as Pickering stabilizers. The resultant emulsions were stable for weeks in ambient conditions, and our platform was challenged using the epitope of the SARS-CoV-2-S1 peptide that served as a model epitope in this study. In vivo assays showed that the αSARS-CoV-2-S1 immunoglobulin G (IgG) titers of the studied mouse antisera, developed against the SARS-CoV-2-S1 peptide under different epitope preparation conditions, showed an order of magnitude higher IgG titers in the studied VLP-based emulsions than epitopes dissolved in water and epitopes administered with an adjuvant, thereby confirming the efficacy of the formulation. This VLP-based Pickering emulsion platform is a fully synthetic approach that can be readily applied for vaccine development to a wide range of pathogens.
AB - The efficiency of epitope-based vaccination (subunit vaccines) is tightly correlated with heterogeneity and the high density of epitope presentation, which maximizes the potential antigenic determinants. Here, we developed a two-mode platform for intensifying the epitope presentation of subunit vaccines. The two-mode epitope presentation enhancement includes a covalent attachment of high concentrations of SARS-CoV-2-S1 peptide epitope to the surface of virus-like-particles (VLPs) and the subsequent assembly of VLP/epitope conjugates on the oil droplet surface at an oil/water interface of an emulsion as Pickering stabilizers. The resultant emulsions were stable for weeks in ambient conditions, and our platform was challenged using the epitope of the SARS-CoV-2-S1 peptide that served as a model epitope in this study. In vivo assays showed that the αSARS-CoV-2-S1 immunoglobulin G (IgG) titers of the studied mouse antisera, developed against the SARS-CoV-2-S1 peptide under different epitope preparation conditions, showed an order of magnitude higher IgG titers in the studied VLP-based emulsions than epitopes dissolved in water and epitopes administered with an adjuvant, thereby confirming the efficacy of the formulation. This VLP-based Pickering emulsion platform is a fully synthetic approach that can be readily applied for vaccine development to a wide range of pathogens.
KW - Pickering emulsion
KW - epitope-based vaccination
KW - plant virus
KW - subunit vaccines
KW - virus-like-particles
UR - http://www.scopus.com/inward/record.url?scp=85135978525&partnerID=8YFLogxK
U2 - 10.1021/acsabm.2c00410
DO - 10.1021/acsabm.2c00410
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C2 - 35913405
AN - SCOPUS:85135978525
SN - 2576-6422
VL - 5
SP - 3859
EP - 3869
JO - ACS Applied Bio Materials
JF - ACS Applied Bio Materials
IS - 8
ER -
