TY - JOUR
T1 - Plugging into enzymes
T2 - Nanowiring of redox enzymes by a gold nanoparticle
AU - Xiao, Yi
AU - Patolsky, Fernando
AU - Katz, Eugenii
AU - Hainfeld, James F.
AU - Willner, Itamar
PY - 2003/3/21
Y1 - 2003/3/21
N2 - The reconstitution of an apo-flavoenzyme, apo-glucose oxidase, on a 1,4-nanometer gold nanocrystal functionalized with the cofactor flavin adenine dinucleotide and integrated into a conductive film yields a bioelectrocatalytic system with exceptional electrical contact with the electrode support. The electron transfer turnover rate of the reconstituted bioelectrocatalyst is ∼5000 per second, compared with the rate at which molecular oxygen, the natural cosubstrate of the enzyme, accepts electrons (∼700 per second). The gold nanoparticle acts as an electron relay or "electrical nanoplug" for the alignment of the enzyme on the conductive support and for the electrical wiring of its redox-active center.
AB - The reconstitution of an apo-flavoenzyme, apo-glucose oxidase, on a 1,4-nanometer gold nanocrystal functionalized with the cofactor flavin adenine dinucleotide and integrated into a conductive film yields a bioelectrocatalytic system with exceptional electrical contact with the electrode support. The electron transfer turnover rate of the reconstituted bioelectrocatalyst is ∼5000 per second, compared with the rate at which molecular oxygen, the natural cosubstrate of the enzyme, accepts electrons (∼700 per second). The gold nanoparticle acts as an electron relay or "electrical nanoplug" for the alignment of the enzyme on the conductive support and for the electrical wiring of its redox-active center.
UR - http://www.scopus.com/inward/record.url?scp=0037459369&partnerID=8YFLogxK
U2 - 10.1126/science.1080664
DO - 10.1126/science.1080664
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C2 - 12649477
AN - SCOPUS:0037459369
SN - 0036-8075
VL - 299
SP - 1877
EP - 1881
JO - Science
JF - Science
IS - 5614
ER -