Pluripotent stem cell miRNAs and metastasis in invasive breast cancer: Journal of the National Cancer Institute

S. Volinia; G. Nuovo; A. Drusco; S. Costinean; R. Abujarour; C. Desponts; M. Garofalo; R. Baffa; R. Aeqilan; K. Maharry; M.E.S.R. Garzon; G. Di Leva; P. Gasparini; P. Dama; J. Marchesini; M. Galasso; M. Manfrini; C. Zerbinati; F. Corrà; T. Wise; S.E. Wojcik; M. Previati; F. Pichiorri; N. Zanesi; H. Alder; J. Palatini; K.F. Huebner; C.L. Shapiro; M. Negrini; A. Vecchione; A.L. Rosenberg; C.M. Croce

doi:10.1093/jnci/dju324

Pluripotent stem cell miRNAs and metastasis in invasive breast cancer: Journal of the National Cancer Institute

S. Volinia, G. Nuovo, A. Drusco, S. Costinean, R. Abujarour, C. Desponts, M. Garofalo, R. Baffa, R. Aeqilan, K. Maharry, M.E.S.R. Garzon, G. Di Leva, P. Gasparini, P. Dama, J. Marchesini, M. Galasso, M. Manfrini, C. Zerbinati, F. Corrà, T. WiseS.E. Wojcik, M. Previati, F. Pichiorri, N. Zanesi, H. Alder, J. Palatini, K.F. Huebner, C.L. Shapiro, M. Negrini, A. Vecchione, A.L. Rosenberg, C.M. Croce

Department of Immunology and Cancer Research

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Background The purpose of this study is to determine whether microRNA for pluripotent stem cells are also expressed in breast cancer and are associated with metastasis and outcome. Methods We studied global microRNA profiles during differentiation of human embryonic stem cells (n =26) and in breast cancer patients (n = 33) and human cell lines (n = 35). Using in situ hybridization, we then investigated MIR302 expression in 318 untreated breast cancer patients (test cohort, n = 22 and validation cohort, n = 296). In parallel, using next-generation sequencing data from breast cancer patients (n = 684), we assessed microRNA association with stem cell markers. All statistical tests were two-sided. Results In healthy tissues, the MIR302 (high)/MIR203 (low) asymmetry was exclusive for pluripotent stem cells. MIR302 was expressed in a small population of cancer cells within invasive ductal carcinoma, but not in normal breast (P

Original language	English
Journal	Journal of the National Cancer Institute
Volume	106
Issue number	12
DOIs	https://doi.org/10.1093/jnci/dju324
State	Published - 2014

Bibliographical note

Export Date: 07 July 2022; Cited By: 27; Correspondence Address: S. Volinia; Deptartment of Morphology, Surgery and Experimental Medicine, LTTA, University of Ferrara, Ferrara, 44121, Italy; email: s.volinia@unife.it; CODEN: JNCIA

Keywords

Breast
Breast Neoplasms
Carcinoma, Ductal, Breast
Female
Humans
Lymphatic Metastasis
MicroRNAs
Neoplastic Stem Cells
Pluripotent Stem Cells
BMI1 protein
Hermes antigen
microRNA
microRNA 203
microRNA 302
unclassified drug
MIRN302 microRNA, human
adult
animal cell
Article
bioinformatics
breast carcinoma
cancer survival
cell differentiation
controlled study
distant metastasis
embryo
embryonic stem cell
female
gene expression
gene repression
genetic association
human
human cell
human tissue
in situ hybridization
lymph node metastasis
major clinical study
male
microarray analysis
middle aged
mouse
next generation sequencing
nonhuman
outcome assessment
overall survival
pluripotent stem cell
polymerase chain reaction
priority journal
survival time
breast
breast tumor
cancer stem cell
genetics
Paget nipple disease
pathology
secondary

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1093/jnci/dju324

https://www.scopus.com/inward/record.uri?eid=2-s2.0-84922391474&doi=10.1093%2fjnci%2fdju324&partnerID=40&md5=3a8ebfbd757423881e1ad3fe07060552

Cite this

Volinia, S., Nuovo, G., Drusco, A., Costinean, S., Abujarour, R., Desponts, C., Garofalo, M., Baffa, R., Aeqilan, R., Maharry, K., Garzon, M. E. S. R., Di Leva, G., Gasparini, P., Dama, P., Marchesini, J., Galasso, M., Manfrini, M., Zerbinati, C., Corrà, F., ... Croce, C. M. (2014). Pluripotent stem cell miRNAs and metastasis in invasive breast cancer: Journal of the National Cancer Institute. Journal of the National Cancer Institute, 106(12). https://doi.org/10.1093/jnci/dju324

@article{2e40ab20690a452ab4c12baa47b36b6d,

title = "Pluripotent stem cell miRNAs and metastasis in invasive breast cancer: Journal of the National Cancer Institute",

abstract = "Background The purpose of this study is to determine whether microRNA for pluripotent stem cells are also expressed in breast cancer and are associated with metastasis and outcome. Methods We studied global microRNA profiles during differentiation of human embryonic stem cells (n =26) and in breast cancer patients (n = 33) and human cell lines (n = 35). Using in situ hybridization, we then investigated MIR302 expression in 318 untreated breast cancer patients (test cohort, n = 22 and validation cohort, n = 296). In parallel, using next-generation sequencing data from breast cancer patients (n = 684), we assessed microRNA association with stem cell markers. All statistical tests were two-sided. Results In healthy tissues, the MIR302 (high)/MIR203 (low) asymmetry was exclusive for pluripotent stem cells. MIR302 was expressed in a small population of cancer cells within invasive ductal carcinoma, but not in normal breast (P ",

keywords = "Breast, Breast Neoplasms, Carcinoma, Ductal, Breast, Female, Humans, Lymphatic Metastasis, MicroRNAs, Neoplastic Stem Cells, Pluripotent Stem Cells, BMI1 protein, Hermes antigen, microRNA, microRNA 203, microRNA 302, unclassified drug, MIRN302 microRNA, human, adult, animal cell, Article, bioinformatics, breast carcinoma, cancer survival, cell differentiation, controlled study, distant metastasis, embryo, embryonic stem cell, female, gene expression, gene repression, genetic association, human, human cell, human tissue, in situ hybridization, lymph node metastasis, major clinical study, male, microarray analysis, middle aged, mouse, next generation sequencing, nonhuman, outcome assessment, overall survival, pluripotent stem cell, polymerase chain reaction, priority journal, survival time, breast, breast tumor, cancer stem cell, genetics, Paget nipple disease, pathology, secondary",

author = "S. Volinia and G. Nuovo and A. Drusco and S. Costinean and R. Abujarour and C. Desponts and M. Garofalo and R. Baffa and R. Aeqilan and K. Maharry and M.E.S.R. Garzon and {Di Leva}, G. and P. Gasparini and P. Dama and J. Marchesini and M. Galasso and M. Manfrini and C. Zerbinati and F. Corr{\`a} and T. Wise and S.E. Wojcik and M. Previati and F. Pichiorri and N. Zanesi and H. Alder and J. Palatini and K.F. Huebner and C.L. Shapiro and M. Negrini and A. Vecchione and A.L. Rosenberg and C.M. Croce",

note = "Export Date: 07 July 2022; Cited By: 27; Correspondence Address: S. Volinia; Deptartment of Morphology, Surgery and Experimental Medicine, LTTA, University of Ferrara, Ferrara, 44121, Italy; email: s.volinia@unife.it; CODEN: JNCIA",

year = "2014",

doi = "10.1093/jnci/dju324",

language = "אנגלית",

volume = "106",

journal = "Journal of the National Cancer Institute",

issn = "0027-8874",

publisher = "Oxford University Press",

number = "12",

}

Volinia, S, Nuovo, G, Drusco, A, Costinean, S, Abujarour, R, Desponts, C, Garofalo, M, Baffa, R, Aeqilan, R, Maharry, K, Garzon, MESR, Di Leva, G, Gasparini, P, Dama, P, Marchesini, J, Galasso, M, Manfrini, M, Zerbinati, C, Corrà, F, Wise, T, Wojcik, SE, Previati, M, Pichiorri, F, Zanesi, N, Alder, H, Palatini, J, Huebner, KF, Shapiro, CL, Negrini, M, Vecchione, A, Rosenberg, AL & Croce, CM 2014, 'Pluripotent stem cell miRNAs and metastasis in invasive breast cancer: Journal of the National Cancer Institute', Journal of the National Cancer Institute, vol. 106, no. 12. https://doi.org/10.1093/jnci/dju324

TY - JOUR

T1 - Pluripotent stem cell miRNAs and metastasis in invasive breast cancer

T2 - Journal of the National Cancer Institute

AU - Volinia, S.

AU - Nuovo, G.

AU - Drusco, A.

AU - Costinean, S.

AU - Abujarour, R.

AU - Desponts, C.

AU - Garofalo, M.

AU - Baffa, R.

AU - Aeqilan, R.

AU - Maharry, K.

AU - Garzon, M.E.S.R.

AU - Di Leva, G.

AU - Gasparini, P.

AU - Dama, P.

AU - Marchesini, J.

AU - Galasso, M.

AU - Manfrini, M.

AU - Zerbinati, C.

AU - Corrà, F.

AU - Wise, T.

AU - Wojcik, S.E.

AU - Previati, M.

AU - Pichiorri, F.

AU - Zanesi, N.

AU - Alder, H.

AU - Palatini, J.

AU - Huebner, K.F.

AU - Shapiro, C.L.

AU - Negrini, M.

AU - Vecchione, A.

AU - Rosenberg, A.L.

AU - Croce, C.M.

N1 - Export Date: 07 July 2022; Cited By: 27; Correspondence Address: S. Volinia; Deptartment of Morphology, Surgery and Experimental Medicine, LTTA, University of Ferrara, Ferrara, 44121, Italy; email: s.volinia@unife.it; CODEN: JNCIA

PY - 2014

Y1 - 2014

N2 - Background The purpose of this study is to determine whether microRNA for pluripotent stem cells are also expressed in breast cancer and are associated with metastasis and outcome. Methods We studied global microRNA profiles during differentiation of human embryonic stem cells (n =26) and in breast cancer patients (n = 33) and human cell lines (n = 35). Using in situ hybridization, we then investigated MIR302 expression in 318 untreated breast cancer patients (test cohort, n = 22 and validation cohort, n = 296). In parallel, using next-generation sequencing data from breast cancer patients (n = 684), we assessed microRNA association with stem cell markers. All statistical tests were two-sided. Results In healthy tissues, the MIR302 (high)/MIR203 (low) asymmetry was exclusive for pluripotent stem cells. MIR302 was expressed in a small population of cancer cells within invasive ductal carcinoma, but not in normal breast (P

AB - Background The purpose of this study is to determine whether microRNA for pluripotent stem cells are also expressed in breast cancer and are associated with metastasis and outcome. Methods We studied global microRNA profiles during differentiation of human embryonic stem cells (n =26) and in breast cancer patients (n = 33) and human cell lines (n = 35). Using in situ hybridization, we then investigated MIR302 expression in 318 untreated breast cancer patients (test cohort, n = 22 and validation cohort, n = 296). In parallel, using next-generation sequencing data from breast cancer patients (n = 684), we assessed microRNA association with stem cell markers. All statistical tests were two-sided. Results In healthy tissues, the MIR302 (high)/MIR203 (low) asymmetry was exclusive for pluripotent stem cells. MIR302 was expressed in a small population of cancer cells within invasive ductal carcinoma, but not in normal breast (P

KW - Breast

KW - Breast Neoplasms

KW - Carcinoma, Ductal, Breast

KW - Female

KW - Humans

KW - Lymphatic Metastasis

KW - MicroRNAs

KW - Neoplastic Stem Cells

KW - Pluripotent Stem Cells

KW - BMI1 protein

KW - Hermes antigen

KW - microRNA

KW - microRNA 203

KW - microRNA 302

KW - unclassified drug

KW - MIRN302 microRNA, human

KW - adult

KW - animal cell

KW - Article

KW - bioinformatics

KW - breast carcinoma

KW - cancer survival

KW - cell differentiation

KW - controlled study

KW - distant metastasis

KW - embryo

KW - embryonic stem cell

KW - female

KW - gene expression

KW - gene repression

KW - genetic association

KW - human

KW - human cell

KW - human tissue

KW - in situ hybridization

KW - lymph node metastasis

KW - major clinical study

KW - male

KW - microarray analysis

KW - middle aged

KW - mouse

KW - next generation sequencing

KW - nonhuman

KW - outcome assessment

KW - overall survival

KW - pluripotent stem cell

KW - polymerase chain reaction

KW - priority journal

KW - survival time

KW - breast

KW - breast tumor

KW - cancer stem cell

KW - genetics

KW - Paget nipple disease

KW - pathology

KW - secondary

UR - http://www.scopus.com/inward/record.url?scp=84922391474&partnerID=8YFLogxK

U2 - 10.1093/jnci/dju324

DO - 10.1093/jnci/dju324

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

SN - 0027-8874

VL - 106

JO - Journal of the National Cancer Institute

JF - Journal of the National Cancer Institute

IS - 12

ER -

Pluripotent stem cell miRNAs and metastasis in invasive breast cancer: Journal of the National Cancer Institute

Abstract

Bibliographical note

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this