Pluripotent stem cell miRNAs and metastasis in invasive breast cancer: Journal of the National Cancer Institute

S. Volinia, G. Nuovo, A. Drusco, S. Costinean, R. Abujarour, C. Desponts, M. Garofalo, R. Baffa, R. Aeqilan, K. Maharry, M.E.S.R. Garzon, G. Di Leva, P. Gasparini, P. Dama, J. Marchesini, M. Galasso, M. Manfrini, C. Zerbinati, F. Corrà, T. WiseS.E. Wojcik, M. Previati, F. Pichiorri, N. Zanesi, H. Alder, J. Palatini, K.F. Huebner, C.L. Shapiro, M. Negrini, A. Vecchione, A.L. Rosenberg, C.M. Croce

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


Background The purpose of this study is to determine whether microRNA for pluripotent stem cells are also expressed in breast cancer and are associated with metastasis and outcome. Methods We studied global microRNA profiles during differentiation of human embryonic stem cells (n =26) and in breast cancer patients (n = 33) and human cell lines (n = 35). Using in situ hybridization, we then investigated MIR302 expression in 318 untreated breast cancer patients (test cohort, n = 22 and validation cohort, n = 296). In parallel, using next-generation sequencing data from breast cancer patients (n = 684), we assessed microRNA association with stem cell markers. All statistical tests were two-sided. Results In healthy tissues, the MIR302 (high)/MIR203 (low) asymmetry was exclusive for pluripotent stem cells. MIR302 was expressed in a small population of cancer cells within invasive ductal carcinoma, but not in normal breast (P
Original languageEnglish
JournalJournal of the National Cancer Institute
Issue number12
StatePublished - 2014

Bibliographical note

Export Date: 07 July 2022; Cited By: 27; Correspondence Address: S. Volinia; Deptartment of Morphology, Surgery and Experimental Medicine, LTTA, University of Ferrara, Ferrara, 44121, Italy; email:; CODEN: JNCIA


  • Breast
  • Breast Neoplasms
  • Carcinoma, Ductal, Breast
  • Female
  • Humans
  • Lymphatic Metastasis
  • MicroRNAs
  • Neoplastic Stem Cells
  • Pluripotent Stem Cells
  • BMI1 protein
  • Hermes antigen
  • microRNA
  • microRNA 203
  • microRNA 302
  • unclassified drug
  • MIRN302 microRNA, human
  • adult
  • animal cell
  • Article
  • bioinformatics
  • breast carcinoma
  • cancer survival
  • cell differentiation
  • controlled study
  • distant metastasis
  • embryo
  • embryonic stem cell
  • female
  • gene expression
  • gene repression
  • genetic association
  • human
  • human cell
  • human tissue
  • in situ hybridization
  • lymph node metastasis
  • major clinical study
  • male
  • microarray analysis
  • middle aged
  • mouse
  • next generation sequencing
  • nonhuman
  • outcome assessment
  • overall survival
  • pluripotent stem cell
  • polymerase chain reaction
  • priority journal
  • survival time
  • breast
  • breast tumor
  • cancer stem cell
  • genetics
  • Paget nipple disease
  • pathology
  • secondary


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