Polyamines and cellular adenosine 3':5'-cyclic monophosphate

C. Clo, C. M. Caldarera, B. Tantini, D. Benalal, U. Bachrach

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18 Scopus citations

Abstract

The effect of polyamines on the cellular concentrations of cyclic AMP was studied. It was shown that 1 μm-spermene caused a decrease in cyclic AMP in chick-embryo heart cells, chick-embryo fibroblasts, neuroblastoma, glioma and neuroblastoma-glioma hybrid cells, grown in culture. A similar decrease was observed when polyamines were added to cells in the presence of a phosphodiesterase inhibitor or after stimulating the cells with various hormones. Noradrenaline was used in cultures of heart cells, prostaglandin E1 and adenosine for neuroblastoma and neuroblastoma-glioma hybrids, whereas isoproterenol was used for the stimulation of glioma cells. Polyamines at higher concentrations were either without effect or caused a slight increase in cyclic AMP. Spermidine (10 μm) also caused a decreased in cellular cyclic AMP, as did 0.1 μm-putrescine. It is suggested that the effect of polyamines on cellular cyclic AMP may be explained by the effect of these polycations on the activity of cellular phosphodiesterase.

Original languageEnglish
Pages (from-to)641-649
Number of pages9
JournalBiochemical Journal
Volume182
Issue number3
DOIs
StatePublished - 1979

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