TY - JOUR
T1 - Polyamines and tumor cells
T2 - Effect of transformation of chick embryo fibroblasts by rous sarcoma virus on polyamine levels
AU - Bachrach, U.
AU - Don, S.
AU - Wiener, H.
PY - 1973/12/10
Y1 - 1973/12/10
N2 - The effect of transformation of chick embryo fibroblasts, by Rous sarcoma virus, on intracellular polyamine levels has been studied. A good correlation between spermidine and cellular protein content has been demonstrated. Upon changing the medium, a sharp increase in spermidine level was noticed both in normal and transformed cells. This increase was accompanied by enhanced protein synthesis. The intracellular concentrations of spermine and spermidine were very similar in normal and transformed cells. On the other hand, significant differences in putrescine levels were demonstrated: in normal cultures the intracellular concentration of putrescine reached a plateau approximately 6 days after seeding, whereas a continuous rise of the diamine in transformed cells was noticed. These differences, which were observed in cultured cells, may explain the known accumulation of polyamines during neoplastic growth.
AB - The effect of transformation of chick embryo fibroblasts, by Rous sarcoma virus, on intracellular polyamine levels has been studied. A good correlation between spermidine and cellular protein content has been demonstrated. Upon changing the medium, a sharp increase in spermidine level was noticed both in normal and transformed cells. This increase was accompanied by enhanced protein synthesis. The intracellular concentrations of spermine and spermidine were very similar in normal and transformed cells. On the other hand, significant differences in putrescine levels were demonstrated: in normal cultures the intracellular concentration of putrescine reached a plateau approximately 6 days after seeding, whereas a continuous rise of the diamine in transformed cells was noticed. These differences, which were observed in cultured cells, may explain the known accumulation of polyamines during neoplastic growth.
UR - http://www.scopus.com/inward/record.url?scp=0015712490&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(73)91246-1
DO - 10.1016/0006-291X(73)91246-1
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C2 - 4357423
AN - SCOPUS:0015712490
SN - 0006-291X
VL - 55
SP - 1035
EP - 1041
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -
