Poly(dG)-poly(dC) DNA appears shorter than poly(dA)-poly(dT) and possibly adopts an A-related conformation on a mica surface under ambient conditions

Natalia Borovok, Tatiana Molotsky, Jamal Ghabboun, Hezy Cohen, Danny Porath*, Alexander Kotlyar

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Three types of DNA: ∼2700 bp polydeoxyguanylic olydeoxycytidylic acid [poly(dG)-poly(dC)], ∼2700 bp polydeoxyadenylic polydeoxythymidylic acid [poly(dA)-poly(dT)] and 2686 bp linear plasmid pUC19 were deposited on a mica surface and imaged by atomic force microscopy. Contour length measurements show that the average length of poly(dG)-poly(dC) is ∼30% shorter than that of poly(dA)-poly(dT) and the plasmid. This led us to suggest that individual poly(dG)-poly(dC) molecules are immobilized on mica under ambient conditions in a form which is likely related to the A-form of DNA in contrast to poly(dA)-poly(dT) and random sequence DNA which are immobilized in a form that is related to the DNA B-form.

Original languageAmerican English
Pages (from-to)5843-5846
Number of pages4
JournalFEBS Letters
Volume581
Issue number30
DOIs
StatePublished - 22 Dec 2007

Bibliographical note

Funding Information:
We thank Igor Brodsky for assistance and fruitful discussions. The work was supported by the EC through Contracts IST-2001-38951 (“DNA-Based Nanowires”) and FP6-029192 (“DNA-Based Nanodevices”).

Keywords

  • A-form
  • Atomic force microscopy (AFM)
  • DNA imaging
  • Poly(dA)-poly(dT)
  • Poly(dG)-poly(dC)

Fingerprint

Dive into the research topics of 'Poly(dG)-poly(dC) DNA appears shorter than poly(dA)-poly(dT) and possibly adopts an A-related conformation on a mica surface under ambient conditions'. Together they form a unique fingerprint.

Cite this