Polymerase chain reaction using noninvasively obtained samples, for the detection of Leishmania infantum DNA in dogs

Dalit Strauss-Ayali*, Charlas L. Jaffe, Ofer Burshtain, Liat Gonen, Gad Baneth

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

122 Scopus citations

Abstract

A polymerase chain reaction (PCR) procedure using noninvasively obtained samples, for the identification of Leishmania infantum in canine tissues, was evaluated and compared with serologic testing and culture. A total of 92% of naturally infected, symptomatic, seropositive dogs were found to be positive by use of DNA from conjunctival swabs. Spleen or lymph node aspirates were found to be positive by PCR in 86% and by culture in 74% of these dogs. The sensitivity and specificity of conjunctival PCR were 92% and 100%, respectively. Experimentally infected dogs were found to be positive by conjunctival PCR already at 45 days of infection (83%) and before seroconversion. PCR using noninvasively obtained conjunctival samples will be useful for epidemiological studies and for direct diagnosis of canine visceral leishmaniasis.

Original languageAmerican English
Pages (from-to)1729-1733
Number of pages5
JournalJournal of Infectious Diseases
Volume189
Issue number9
DOIs
StatePublished - 1 May 2004

Bibliographical note

Funding Information:
Financial support: Deutsche Forschungsgemeinschaft for the Palestinian-German-Israeli Cooperative Project on Leishmaniosis in Palestine and Israel (grant SO 220/ 5-1); Center for the Study of Emerging Diseases (grant support); US-Israel Cooperative Development Research Program, Economic Growth, US Agency for International Development (grant TA-MOU-00-C20-025); Yeshaya Horowitz Foundation (fellowship to D.S.-A.).

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