Poly(vinyl alcohol)-methacrylate with CRGD peptide: A photocurable biocompatible hydrogel

Michael Goldvaser, Eyal Epstein, Osnat Rosen, Avital Jayson, Niva Natan, Tal Ben-Shalom, Sigal Saphier, Shahaf Katalan, Oded Shoseyov*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Polyvinyl alcohol (PVA)-based hydrogels are promising biomaterials for tissue engineering printing applications. However, one of their main disadvantages is their inability to support cell attachment, which is a critical feature for the preparation of biological scaffolds. The goal of this study was to develop a printable, cell-supportive PVA-based bioink with tunable mechanical properties, without using animal-derived polymers which potentially harbor human pathogens. An ultraviolet light (UV) curable PVA-methacrylate (PVA-MA) polymer mixed with Cys-Arg-Gly-Asp (CRGD) peptide was developed. This peptide holds the integrin receptor binding sequence – RGD, that can enhance cell attachment. The additional cysteine was designed to enable its thiol binding under UV to methacrylate groups of the UV curable PVA-MA. Vero cell, as an adherent cell model was used to assess the hydrogel's cell adhesion. It was found that the PVA-MA-CRGD formula enables the preparation of hydrogels with excellent cell attachment and had even shown superior cell attachment properties relative to added gelatin. Adding hyaluronic acid (HA) as a rheologic modulator enabled the printing of this new formula. Our overall data demonstrates the applicability of this mixture as a bioink for soft tissue engineering such as skin, adipose, liver or kidney tissue.

Original languageAmerican English
Pages (from-to)140-150
Number of pages11
JournalJournal of Tissue Engineering and Regenerative Medicine
Issue number2
StatePublished - Feb 2022

Bibliographical note

Funding Information:
The authors thank Meni Girshengorn and Lilach Levin for their proficient support, and for Dr. Avital Vaknin and Dr. Yehudit Posen for editorial assistance.

Publisher Copyright:
© 2021 John Wiley & Sons Ltd.

© 2021 John Wiley & Sons Ltd.


  • Animals
  • Biocompatible Materials/chemistry
  • Hydrogels/chemistry
  • Methacrylates/chemistry
  • Peptides, Cyclic
  • Polyvinyl Alcohol/chemistry
  • Tissue Engineering
  • Humans


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