TY - JOUR
T1 - Position effect variegations and brain-specific silencing in transgenic mice overexpressing human acetylcholinesterase variants
AU - Sternfeld, Meira
AU - Patrick, James D.
AU - Soreq, Hermona
PY - 1998
Y1 - 1998
N2 - Position effect variegations as well as brain-specific silencing were observed in novel transgenic mouse pedigrees expressing human acetylcholinesterase (AChE) variants. Muscle AChE activities varied between 1.6- and 350-fold of control in these lines, one carrying insertion- inactivated lnE6-AChE and two with 'readthrough' I4/E5 AChE, all under control of the ubiquitous CMV promoter. In contrast, brain AChE levels remained within a range of 1.5-fold over control, suggesting an upper limit of brain AChE which is compatible with life.
AB - Position effect variegations as well as brain-specific silencing were observed in novel transgenic mouse pedigrees expressing human acetylcholinesterase (AChE) variants. Muscle AChE activities varied between 1.6- and 350-fold of control in these lines, one carrying insertion- inactivated lnE6-AChE and two with 'readthrough' I4/E5 AChE, all under control of the ubiquitous CMV promoter. In contrast, brain AChE levels remained within a range of 1.5-fold over control, suggesting an upper limit of brain AChE which is compatible with life.
UR - http://www.scopus.com/inward/record.url?scp=0032105403&partnerID=8YFLogxK
U2 - 10.1016/S0928-4257(98)80028-4
DO - 10.1016/S0928-4257(98)80028-4
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C2 - 9789818
AN - SCOPUS:0032105403
SN - 0928-4257
VL - 92
SP - 249
EP - 255
JO - Journal of Physiology Paris
JF - Journal of Physiology Paris
IS - 3-4
ER -