TY - JOUR
T1 - Positive correlation between superoxide dismutase and resistance to paraquat toxicity in the green alga Chlorella sorokiniana
AU - Rabinowitch, Haim D.
AU - Clare, Debra A.
AU - Crapo, James D.
AU - Fridovich, Irwin
PY - 1983/9
Y1 - 1983/9
N2 - Paraquat (10-30 μm) exerted a dose-dependent and light-dependent toxicity on Chlorella sorokiniana. Paraquat was also seen to increase the superoxide dismutase content of these cells and to cause the appearance of a new electrophoretically distinct isozyme. Cells grown in the absence of paraquat contained one manganese-superoxide dismutase and two iron-superoxide dismutases, while the paraquat-grown cells contained an additional manganese-superoxide dismutase. Cells which were grown in the presence of 25 μm Paraquat, and which therefore possessed elevated levels of superoxide dismutase, were resistant to 30 μm Paraquat, whereas control cells were bleached and killed by this level of Paraquat. Electron micrography and chemical analysis revealed that Paraquat decreased the starch content of the cells and caused a failure of dividing cells to separate. It appears that Paraquat increases the photoproduction of O2- in C. sorokiniana and that an increase in the cell content of superoxide dismutase is an adaptive response which provides protection against this herbicide.
AB - Paraquat (10-30 μm) exerted a dose-dependent and light-dependent toxicity on Chlorella sorokiniana. Paraquat was also seen to increase the superoxide dismutase content of these cells and to cause the appearance of a new electrophoretically distinct isozyme. Cells grown in the absence of paraquat contained one manganese-superoxide dismutase and two iron-superoxide dismutases, while the paraquat-grown cells contained an additional manganese-superoxide dismutase. Cells which were grown in the presence of 25 μm Paraquat, and which therefore possessed elevated levels of superoxide dismutase, were resistant to 30 μm Paraquat, whereas control cells were bleached and killed by this level of Paraquat. Electron micrography and chemical analysis revealed that Paraquat decreased the starch content of the cells and caused a failure of dividing cells to separate. It appears that Paraquat increases the photoproduction of O2- in C. sorokiniana and that an increase in the cell content of superoxide dismutase is an adaptive response which provides protection against this herbicide.
UR - http://www.scopus.com/inward/record.url?scp=0020627128&partnerID=8YFLogxK
U2 - 10.1016/0003-9861(83)90075-9
DO - 10.1016/0003-9861(83)90075-9
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C2 - 6625604
AN - SCOPUS:0020627128
SN - 0003-9861
VL - 225
SP - 640
EP - 648
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 2
ER -