TY - JOUR
T1 - Possible involvement of lipoxygenase products of arachidonic acid pathway in ovulation
AU - Reich, R.
AU - Kohen, F.
AU - Naor, Z.
AU - Tsafriri, A.
PY - 1983/12
Y1 - 1983/12
N2 - The possible involvement of products of the lipoxygenase pathway of archidonic acid cascade in ovulation was tested by intrabursal injection of nordihydroguaiaretic acid (NDGA); 5,8,11-eicosatriynoic acid (5,8,11-ETYA), 3 amino-1-(3 trifluromethyphenyl)-2-pyrazoline hydrochloride (BW755c) and (FPL 55712). All these drugs reduced the number of ova released from the treated ovaries in a dose-dependent manner, without affecting ovulation from contralateral ovaries. NDGA was most potent since it completely blocked ovulation from the treated ovaried in 17/38 rats receiving a dose higher than 0.15 mg/ bursa. This effect og NDGA cannot be ascribed to its inhibition of ovarian PGE synthesis. Conversion of labeled arachidonic acid and via the lipoxygenase pathway by preovulatory rat follicles was demonstrated by TLC chromatography. Collectively, these results suggest the involvement of products of lipoxygenase pathway of arachidonic acid in ovulation in the rat.
AB - The possible involvement of products of the lipoxygenase pathway of archidonic acid cascade in ovulation was tested by intrabursal injection of nordihydroguaiaretic acid (NDGA); 5,8,11-eicosatriynoic acid (5,8,11-ETYA), 3 amino-1-(3 trifluromethyphenyl)-2-pyrazoline hydrochloride (BW755c) and (FPL 55712). All these drugs reduced the number of ova released from the treated ovaries in a dose-dependent manner, without affecting ovulation from contralateral ovaries. NDGA was most potent since it completely blocked ovulation from the treated ovaried in 17/38 rats receiving a dose higher than 0.15 mg/ bursa. This effect og NDGA cannot be ascribed to its inhibition of ovarian PGE synthesis. Conversion of labeled arachidonic acid and via the lipoxygenase pathway by preovulatory rat follicles was demonstrated by TLC chromatography. Collectively, these results suggest the involvement of products of lipoxygenase pathway of arachidonic acid in ovulation in the rat.
UR - http://www.scopus.com/inward/record.url?scp=0021021839&partnerID=8YFLogxK
U2 - 10.1016/0090-6980(83)90161-2
DO - 10.1016/0090-6980(83)90161-2
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C2 - 6441969
AN - SCOPUS:0021021839
SN - 0090-6980
VL - 26
SP - 1011
EP - 1020
JO - Prostaglandins
JF - Prostaglandins
IS - 6
ER -