TY - JOUR
T1 - PRAME (preferentially expressed antigen of melanoma) is a novel marker for differentiating serous carcinoma from malignant mesothelioma
AU - Brenne, Kjersti
AU - Nymoen, Dag André
AU - Reich, Reuven
AU - Davidson, Ben
PY - 2012/2
Y1 - 2012/2
N2 - The PRAME (preferentially expressed antigen of melanoma) gene was previously shown to be overexpressed in ovarian/primary peritoneal serous carcinoma compared with malignant mesothelioma using gene expression arrays. The objective of this study was to validate this finding at the messenger RNA (mRNA) and protein levels. Quantitative real-time polymerase chain reaction analysis of 126 müllerian carcinomas and 23 malignant mesotheliomas showed significantly higher PRAME mRNA expression in the former tumor (P < .001; test sensitivity and specificity, 89% and 91%, respectively). PRAME protein was expressed in 41 of 50 müllerian carcinomas and 0 of 30 mesotheliomas usin g Western blotting (P < .001; test sensitivity and specificity, 82% and 100%, respectively). PRAME levels in müllerian carcinoma were unrelated to survival; however, PRAME protein expression was up-regulated in solid metastases compared with primary carcinoma and effusions (P < .001). Our data confirm that PRAME effectively differentiates müllerian carcinoma from malignant mesothelioma at the mRNA and protein levels, suggesting a role in the diagnostic workup of serosal cancers.
AB - The PRAME (preferentially expressed antigen of melanoma) gene was previously shown to be overexpressed in ovarian/primary peritoneal serous carcinoma compared with malignant mesothelioma using gene expression arrays. The objective of this study was to validate this finding at the messenger RNA (mRNA) and protein levels. Quantitative real-time polymerase chain reaction analysis of 126 müllerian carcinomas and 23 malignant mesotheliomas showed significantly higher PRAME mRNA expression in the former tumor (P < .001; test sensitivity and specificity, 89% and 91%, respectively). PRAME protein was expressed in 41 of 50 müllerian carcinomas and 0 of 30 mesotheliomas usin g Western blotting (P < .001; test sensitivity and specificity, 82% and 100%, respectively). PRAME levels in müllerian carcinoma were unrelated to survival; however, PRAME protein expression was up-regulated in solid metastases compared with primary carcinoma and effusions (P < .001). Our data confirm that PRAME effectively differentiates müllerian carcinoma from malignant mesothelioma at the mRNA and protein levels, suggesting a role in the diagnostic workup of serosal cancers.
KW - Diagnosis
KW - Malignant mesothelioma
KW - Müllerian carcinoma
KW - PRAME
KW - Preferentially expressed antigen of melanoma
KW - Quantitative real-time PCR
KW - Serous effusions
KW - Western blotting
UR - http://www.scopus.com/inward/record.url?scp=84856401870&partnerID=8YFLogxK
U2 - 10.1309/AJCPGA95KVSAUDMF
DO - 10.1309/AJCPGA95KVSAUDMF
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C2 - 22261449
AN - SCOPUS:84856401870
SN - 0002-9173
VL - 137
SP - 240
EP - 247
JO - American Journal of Clinical Pathology
JF - American Journal of Clinical Pathology
IS - 2
ER -