Precise measurement of the b2 deletion in coliphage lambda

Michael Fiandt, Alik Honigman, Eric C. Rosenvold, Waclaw Szybalski*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

The length of the b2 deletion is 12.0 ± 0.4%λ, as determined by comparison of the electron micrographically measured lengths of the b2+-containing AvaI fragment 3 from phage λ DNA and the corresponding fragment 3′ from λb2. This is equal to approx. 5700 base pairs, when the λ length is calculated as 48 000 base pairs, based on the current sequence data for phage φX174 and the φX174-RFII/λ DNA length ratio.

Original languageEnglish
Pages (from-to)289-293
Number of pages5
JournalGene
Volume2
Issue number5-6
DOIs
StatePublished - Dec 1977
Externally publishedYes

Keywords

  • AvalI restriction endonuclease
  • Bacteriophage λ
  • electron microscopy of DNA
  • λ/φX174 length ratio

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