TY - JOUR
T1 - Precise measurement of the b2 deletion in coliphage lambda
AU - Fiandt, Michael
AU - Honigman, Alik
AU - Rosenvold, Eric C.
AU - Szybalski, Waclaw
PY - 1977/12
Y1 - 1977/12
N2 - The length of the b2 deletion is 12.0 ± 0.4%λ, as determined by comparison of the electron micrographically measured lengths of the b2+-containing AvaI fragment 3 from phage λ DNA and the corresponding fragment 3′ from λb2. This is equal to approx. 5700 base pairs, when the λ length is calculated as 48 000 base pairs, based on the current sequence data for phage φX174 and the φX174-RFII/λ DNA length ratio.
AB - The length of the b2 deletion is 12.0 ± 0.4%λ, as determined by comparison of the electron micrographically measured lengths of the b2+-containing AvaI fragment 3 from phage λ DNA and the corresponding fragment 3′ from λb2. This is equal to approx. 5700 base pairs, when the λ length is calculated as 48 000 base pairs, based on the current sequence data for phage φX174 and the φX174-RFII/λ DNA length ratio.
KW - AvalI restriction endonuclease
KW - Bacteriophage λ
KW - electron microscopy of DNA
KW - λ/φX174 length ratio
UR - http://www.scopus.com/inward/record.url?scp=0017583927&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(77)90007-5
DO - 10.1016/0378-1119(77)90007-5
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AN - SCOPUS:0017583927
SN - 0378-1119
VL - 2
SP - 289
EP - 293
JO - Gene
JF - Gene
IS - 5-6
ER -