TY - JOUR
T1 - Predominant role of α4-integrins for distinct steps of lymphoma metastasis
AU - Gosslar, Uwe
AU - Jonas, Petra
AU - Luz, Arne
AU - Lifka, Alexandra
AU - Naor, David
AU - Hamann, Alf
AU - Holzmann, Bernhard
PY - 1996/5/14
Y1 - 1996/5/14
N2 - To analyze the role of α4-integrins in lymphoma metastasis, sublines of the T-cell lymphoma LB were generated by retrovirus-mediated gene transfer that differ exclusively in the expression of α4-integrins. Using LB-α4 and control LB-NTK cells, we demonstrate that expression of α4-integrins strongly suppresses metastasis formation of LB lymphoma cells in secondary lymphoid organs such as spleen, mesenteric and peripheral lymph nodes, or Peyer's patches after i.v. injection into syngeneic BALB/c mice. Moreover, α4-integrin expression inhibited development of metastatic tumors in liver, lung, and kidney. Expansion of LB lymphoma cells in bone marrow was not affected by α4-integrin expression. In vivo migration assays using 51Cr- labeled lymphoma cells demonstrated that low-metastatic LB-α4 cells accumulated with the same efficiency as high-metastatic LB-NTK cells in all target organs examined and were even enriched in mucosal lymphoid organs. Collectively, these results indicate that α4-integrins inhibit metastasis formation of lymphoma cells at a stage subsequent to the invasion of target organs.
AB - To analyze the role of α4-integrins in lymphoma metastasis, sublines of the T-cell lymphoma LB were generated by retrovirus-mediated gene transfer that differ exclusively in the expression of α4-integrins. Using LB-α4 and control LB-NTK cells, we demonstrate that expression of α4-integrins strongly suppresses metastasis formation of LB lymphoma cells in secondary lymphoid organs such as spleen, mesenteric and peripheral lymph nodes, or Peyer's patches after i.v. injection into syngeneic BALB/c mice. Moreover, α4-integrin expression inhibited development of metastatic tumors in liver, lung, and kidney. Expansion of LB lymphoma cells in bone marrow was not affected by α4-integrin expression. In vivo migration assays using 51Cr- labeled lymphoma cells demonstrated that low-metastatic LB-α4 cells accumulated with the same efficiency as high-metastatic LB-NTK cells in all target organs examined and were even enriched in mucosal lymphoid organs. Collectively, these results indicate that α4-integrins inhibit metastasis formation of lymphoma cells at a stage subsequent to the invasion of target organs.
UR - http://www.scopus.com/inward/record.url?scp=0029953938&partnerID=8YFLogxK
U2 - 10.1073/pnas.93.10.4821
DO - 10.1073/pnas.93.10.4821
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C2 - 8643487
AN - SCOPUS:0029953938
SN - 0027-8424
VL - 93
SP - 4821
EP - 4826
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 10
ER -