Abstract
Coupling factor 6 (F6) and mitochondrial ATPase inhibitor were isolated from the rutamycin-sensitive ATPase complex of bovine heart mitochondria by heating and fractionation with ethanol. F6 appeared in acrylamide gel electrophoresis in the presence of sodium dodecylsulfate and urea as a single band corresponding to a molecular weight of 8,000. This protein which is required for the 32Pi-ATP exchange in submitochondrial particles treated with silicotungstate was very sensitive to trypsin.
Original language | English |
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Pages (from-to) | 1050-1056 |
Number of pages | 7 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 69 |
Issue number | 4 |
DOIs | |
State | Published - 19 Apr 1976 |
Externally published | Yes |