Preparation and characterization of homogeneous coupling factor 6 from bovine heart mitochondria

Baruch I. Kanner*, Ramon Serrano, M. Anne Kandrach, Efraim Racker

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

65 Scopus citations

Abstract

Coupling factor 6 (F6) and mitochondrial ATPase inhibitor were isolated from the rutamycin-sensitive ATPase complex of bovine heart mitochondria by heating and fractionation with ethanol. F6 appeared in acrylamide gel electrophoresis in the presence of sodium dodecylsulfate and urea as a single band corresponding to a molecular weight of 8,000. This protein which is required for the 32Pi-ATP exchange in submitochondrial particles treated with silicotungstate was very sensitive to trypsin.

Original languageEnglish
Pages (from-to)1050-1056
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume69
Issue number4
DOIs
StatePublished - 19 Apr 1976
Externally publishedYes

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