Preparation and characterization of homogeneous coupling factor 6 from bovine heart mitochondria

Baruch I. Kanner; Ramon Serrano; M. Anne Kandrach; Efraim Racker

doi:10.1016/0006-291X(76)90479-4

Preparation and characterization of homogeneous coupling factor 6 from bovine heart mitochondria

Baruch I. Kanner^*
, Ramon Serrano
, M. Anne Kandrach
, Efraim Racker

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

Coupling factor 6 (F₆) and mitochondrial ATPase inhibitor were isolated from the rutamycin-sensitive ATPase complex of bovine heart mitochondria by heating and fractionation with ethanol. F₆ appeared in acrylamide gel electrophoresis in the presence of sodium dodecylsulfate and urea as a single band corresponding to a molecular weight of 8,000. This protein which is required for the ³²P_i-ATP exchange in submitochondrial particles treated with silicotungstate was very sensitive to trypsin.

Original language	English
Pages (from-to)	1050-1056
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	69
Issue number	4
DOIs	https://doi.org/10.1016/0006-291X(76)90479-4
State	Published - 19 Apr 1976
Externally published	Yes

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10.1016/0006-291X(76)90479-4

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title = "Preparation and characterization of homogeneous coupling factor 6 from bovine heart mitochondria",

abstract = "Coupling factor 6 (F6) and mitochondrial ATPase inhibitor were isolated from the rutamycin-sensitive ATPase complex of bovine heart mitochondria by heating and fractionation with ethanol. F6 appeared in acrylamide gel electrophoresis in the presence of sodium dodecylsulfate and urea as a single band corresponding to a molecular weight of 8,000. This protein which is required for the 32Pi-ATP exchange in submitochondrial particles treated with silicotungstate was very sensitive to trypsin.",

author = "Kanner, \{Baruch I.\} and Ramon Serrano and \{Anne Kandrach\}, M. and Efraim Racker",

year = "1976",

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T1 - Preparation and characterization of homogeneous coupling factor 6 from bovine heart mitochondria

AU - Kanner, Baruch I.

AU - Serrano, Ramon

AU - Anne Kandrach, M.

AU - Racker, Efraim

PY - 1976/4/19

Y1 - 1976/4/19

N2 - Coupling factor 6 (F6) and mitochondrial ATPase inhibitor were isolated from the rutamycin-sensitive ATPase complex of bovine heart mitochondria by heating and fractionation with ethanol. F6 appeared in acrylamide gel electrophoresis in the presence of sodium dodecylsulfate and urea as a single band corresponding to a molecular weight of 8,000. This protein which is required for the 32Pi-ATP exchange in submitochondrial particles treated with silicotungstate was very sensitive to trypsin.

AB - Coupling factor 6 (F6) and mitochondrial ATPase inhibitor were isolated from the rutamycin-sensitive ATPase complex of bovine heart mitochondria by heating and fractionation with ethanol. F6 appeared in acrylamide gel electrophoresis in the presence of sodium dodecylsulfate and urea as a single band corresponding to a molecular weight of 8,000. This protein which is required for the 32Pi-ATP exchange in submitochondrial particles treated with silicotungstate was very sensitive to trypsin.

UR - https://www.scopus.com/pages/publications/0017113598

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

Preparation and characterization of homogeneous coupling factor 6 from bovine heart mitochondria

Abstract

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