Presentation of a three-banded allele pattern - Analysis and interpretation

A. Azmir, M. Shpitzen, C. Oz, U. Motro, V. Meiner, R. Gafny

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

The Israel police forensic biology laboratory received as an item of evidence in an attempted murder case, a pair of trousers belonging to a suspect. A bloodstain was observed on the trousers and analyzed by STR typing for nine loci using the Promega GenePrint™ STR silver stain detection kits. The genetic profile defined was found to be identical to that of the victim's at all nine loci. Within this profile a three-banded allele pattern was observed at the D16S539 locus, both in the bloodstain and in the victim's reference blood sample. Confirmation of this phenomenon was accomplished by amplifying the extracted DNA from both the trousers and the victim's blood sample using the PowerPlex 16 kit by Promega and the AmpF STR SGM Plus kit by Perkin Elmer. followed by analysis of the amplification products by capillary electrophoresis on the ABI prism 310 genetic analyzer. The same three-banded allele pattern was observed at the D16S539 locus in both specimen and reference DNA, using each of the three kits. Three additional loci located on chromosome 16 (D16S3407, D16S2617 and D16S3082), not employed for forensic identification, were also analyzed and did not show three-banded allele pattern.

Original languageEnglish
Pages (from-to)824-826
Number of pages3
JournalJournal of Forensic Sciences
Volume47
Issue number4
StatePublished - 2002

Keywords

  • Bloodstain
  • D16S539
  • DNA typing
  • Forensic science
  • Polymerase chain reaction
  • Short tandem repeats
  • Silver staining

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