Prevalence and molecular characterization of Hepatozoon canis in dogs from urban and rural areas in Southeast Brazil

R. L. de Miranda; L. H. O'Dwyer; J. R. de Castro; B. Metzger; A. S. Rubini; A. V. Mundim; O. Eyal; D. Talmi-Frank; M. C. Cury; G. Baneth

doi:10.1016/j.rvsc.2014.06.015

Prevalence and molecular characterization of Hepatozoon canis in dogs from urban and rural areas in Southeast Brazil

R. L. de Miranda, L. H. O'Dwyer, J. R. de Castro, B. Metzger, A. S. Rubini, A. V. Mundim, O. Eyal, D. Talmi-Frank, M. C. Cury^*, G. Baneth

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

The objective of this survey was to investigate the prevalence of Hepatozoon infection in dogs in the rural and urban areas of Uberlândia, Brazil by PCR and molecular characterization. DNA was obtained from blood samples collected from 346 local dogs from both genders and various ages. Seventeen PCR products from positive blood samples of urban dogs and 13 from the rural dogs were sequenced. Partial sequences of the 18S rRNA gene indicated that all 30 dogs were infected with Hepatozoon canis similar in sequence to H. canis from southern Europe. Four local dog sequences were submitted to GenBank (accessions JN835188; KF692038; KF692039; KF692040). This study indicates that H. canis is the cause of canine hepatozoonosis in Uberlândia and that infection is similarly widespread in rural and urban dogs.

Original language	English
Pages (from-to)	325-328
Number of pages	4
Journal	Research in Veterinary Science
Volume	97
Issue number	2
DOIs	https://doi.org/10.1016/j.rvsc.2014.06.015
State	Published - 2014
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2014 Published by Elsevier Ltd.

Keywords

Brazil
Dogs
Hepatozoonosis
PCR
Prevalence

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10.1016/j.rvsc.2014.06.015

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title = "Prevalence and molecular characterization of Hepatozoon canis in dogs from urban and rural areas in Southeast Brazil",

abstract = "The objective of this survey was to investigate the prevalence of Hepatozoon infection in dogs in the rural and urban areas of Uberl{\^a}ndia, Brazil by PCR and molecular characterization. DNA was obtained from blood samples collected from 346 local dogs from both genders and various ages. Seventeen PCR products from positive blood samples of urban dogs and 13 from the rural dogs were sequenced. Partial sequences of the 18S rRNA gene indicated that all 30 dogs were infected with Hepatozoon canis similar in sequence to H. canis from southern Europe. Four local dog sequences were submitted to GenBank (accessions JN835188; KF692038; KF692039; KF692040). This study indicates that H. canis is the cause of canine hepatozoonosis in Uberl{\^a}ndia and that infection is similarly widespread in rural and urban dogs.",

keywords = "Brazil, Dogs, Hepatozoonosis, PCR, Prevalence",

author = "{de Miranda}, {R. L.} and O'Dwyer, {L. H.} and {de Castro}, {J. R.} and B. Metzger and Rubini, {A. S.} and Mundim, {A. V.} and O. Eyal and D. Talmi-Frank and Cury, {M. C.} and G. Baneth",

note = "Publisher Copyright: {\textcopyright} 2014 Published by Elsevier Ltd.",

year = "2014",

doi = "10.1016/j.rvsc.2014.06.015",

language = "אנגלית",

volume = "97",

pages = "325--328",

journal = "Research in Veterinary Science",

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AU - de Miranda, R. L.

AU - O'Dwyer, L. H.

AU - de Castro, J. R.

AU - Metzger, B.

AU - Rubini, A. S.

AU - Mundim, A. V.

AU - Eyal, O.

AU - Talmi-Frank, D.

AU - Cury, M. C.

AU - Baneth, G.

PY - 2014

Y1 - 2014

N2 - The objective of this survey was to investigate the prevalence of Hepatozoon infection in dogs in the rural and urban areas of Uberlândia, Brazil by PCR and molecular characterization. DNA was obtained from blood samples collected from 346 local dogs from both genders and various ages. Seventeen PCR products from positive blood samples of urban dogs and 13 from the rural dogs were sequenced. Partial sequences of the 18S rRNA gene indicated that all 30 dogs were infected with Hepatozoon canis similar in sequence to H. canis from southern Europe. Four local dog sequences were submitted to GenBank (accessions JN835188; KF692038; KF692039; KF692040). This study indicates that H. canis is the cause of canine hepatozoonosis in Uberlândia and that infection is similarly widespread in rural and urban dogs.

AB - The objective of this survey was to investigate the prevalence of Hepatozoon infection in dogs in the rural and urban areas of Uberlândia, Brazil by PCR and molecular characterization. DNA was obtained from blood samples collected from 346 local dogs from both genders and various ages. Seventeen PCR products from positive blood samples of urban dogs and 13 from the rural dogs were sequenced. Partial sequences of the 18S rRNA gene indicated that all 30 dogs were infected with Hepatozoon canis similar in sequence to H. canis from southern Europe. Four local dog sequences were submitted to GenBank (accessions JN835188; KF692038; KF692039; KF692040). This study indicates that H. canis is the cause of canine hepatozoonosis in Uberlândia and that infection is similarly widespread in rural and urban dogs.

KW - Brazil

KW - Dogs

KW - Hepatozoonosis

KW - PCR

KW - Prevalence

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SN - 0034-5288

VL - 97

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EP - 328

JO - Research in Veterinary Science

JF - Research in Veterinary Science

IS - 2

ER -

Prevalence and molecular characterization of Hepatozoon canis in dogs from urban and rural areas in Southeast Brazil

Abstract

Bibliographical note

Keywords

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