Prevalence and molecular characterization of Hepatozoon canis in dogs from urban and rural areas in Southeast Brazil

R. L. de Miranda, L. H. O'Dwyer, J. R. de Castro, B. Metzger, A. S. Rubini, A. V. Mundim, O. Eyal, D. Talmi-Frank, M. C. Cury*, G. Baneth

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

The objective of this survey was to investigate the prevalence of Hepatozoon infection in dogs in the rural and urban areas of Uberlândia, Brazil by PCR and molecular characterization. DNA was obtained from blood samples collected from 346 local dogs from both genders and various ages. Seventeen PCR products from positive blood samples of urban dogs and 13 from the rural dogs were sequenced. Partial sequences of the 18S rRNA gene indicated that all 30 dogs were infected with Hepatozoon canis similar in sequence to H. canis from southern Europe. Four local dog sequences were submitted to GenBank (accessions JN835188; KF692038; KF692039; KF692040). This study indicates that H. canis is the cause of canine hepatozoonosis in Uberlândia and that infection is similarly widespread in rural and urban dogs.

Original languageAmerican English
Pages (from-to)325-328
Number of pages4
JournalResearch in Veterinary Science
Volume97
Issue number2
DOIs
StatePublished - 2014
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2014 Published by Elsevier Ltd.

Keywords

  • Brazil
  • Dogs
  • Hepatozoonosis
  • PCR
  • Prevalence

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