Prevention of oxidative damage in fibroblast cell cultures and rat skin by positively-charged submicron emulsion of α-tocopherol

R. Ezra, S. Benita*, I. Ginsburg, R. Kohen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


An attempt was made to incorporate α-tocopherol in negatively and positively-charged submicron emulsions, with the aim of providing an effective topical preparation against skin oxidative damage. In cell culture toxicity experiments using human fibroblast it was shown that the positively-charged α-tocopherol emulsion did not exhibit any toxic effect despite the low dilution and respective high concentration used. Negatively and positively-charged submicron emulsions of α-tocopherol and their respective blank emulsions were topically applied to rats that were subjected to UVA irradiation under different experimental conditions. No difference was observed between the negatively and positively-charged α-tocopherol submicron emulsions regarding the rate of oxidation and peroxyl radical scavenging ability of skin homogenates and both were able to protect rat skin against oxidative stress. However, in a non-invasive evaluation of the lipid hydroperoxidation process in rat skin following exposure to UVA irradiation, the positively-charged α-tocopherol submicron emulsion elicited a significantly better protective effect than the corresponding negatively-charged emulsion. These results suggest that the positively-charged emulsion exhibits a more prolonged residence time in the uppermost layers of the skin than the negatively-charged emulsion.

Original languageAmerican English
Pages (from-to)291-298
Number of pages8
JournalEuropean Journal of Pharmaceutics and Biopharmaceutics
Issue number4
StatePublished - 1996


  • Anti-oxidant
  • Emulsion
  • Non-invasive method
  • Oxidation
  • Positive charge
  • Submicron
  • α-Tocopherol


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