Prevention of oxidative damage in the rat jejunal mucosa by pectin

Ron Kohen, Vered Shadmi, Angel Kakunda, Abraham Rubinstein

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

The role of the soluble non-starch polysaccharide pectin in the prevention of oxidative damage induced by peroxy, superoxide and hydroxyl radicals to the rat jejunal mucosa was studied. The oxidative stress was introduced to the rat jejunal mucosa by means of a closed-loop perfusion system and was characterized biochemically by monitoring the enterocyte activity of the enzyme lactate dehydrogenase (EC 1.1.1.27) and the K+ level. Aqueous solutions of pectin were perfused into the rat jejunum before the oxidative stress inducers. The possible protection effect was evaluated by comparing the mucosal integrity (as measured by biochemical variables) to the values obtained after perfusion with the oxidative stress inducers only. We found that: (a) mucosal damage was detected following the perfusion of peroxy and hydroxyl radicals in the rat jejunum, but not following perfusion of the superoxide radical; (b) a significant reduction in the mucosal damage was noted when pectin was perfused before the perfusion with the peroxy radical induction; (c) full protection against the mucosal damage induced by hydroxyl radicals was achieved when pectin was perfused before the damage induction.

Original languageAmerican English
Pages (from-to)789-800
Number of pages12
JournalBritish Journal of Nutrition
Volume69
Issue number3
DOIs
StatePublished - May 1993

Bibliographical note

Funding Information:
This work was supported by a research grant from the Israel Cancer Association (38/90), PO Box 2001 8, Tel Aviv, Israel. It is included in the dissertation project of V. S. as partial fulfilment of the MSc degree requirements of the Hebrew University of Jerusalem.

Keywords

  • Antioxidants
  • Free radicals
  • Mucosal damage
  • Oxidative stress
  • Pectin

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