Probing of DNA and single-base mismatches by chemical force microscopy using peptide nucleic acid-modified sensing tips and functionalized surfaces

Oleg Lioubashevski; Fernando Patolsky; Itamar Willner

doi:10.1021/la0105174

Probing of DNA and single-base mismatches by chemical force microscopy using peptide nucleic acid-modified sensing tips and functionalized surfaces

Oleg Lioubashevski, Fernando Patolsky, Itamar Willner^*

^*Corresponding author for this work

Institute of Chemistry

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

DNA hybridization to peptide nucleic acid (PNA)-functionalized Au-coated tips is characterized by probing the force interactions between the PNA-functionalized tip or the DNA/PNA-functionalized tip with a hydrophobic 1-mercaptoundecane-modified Au surface, using chemical force microscopy (CFM). DNA concentrations as low as 3 × 10^-13 M can be detected by probing the respective force interactions. The CFM method is sufficiently sensitive to detect a single base mismatch in the analyte DNA.

Original language	English
Pages (from-to)	5134-5136
Number of pages	3
Journal	Langmuir
Volume	17
Issue number	17
DOIs	https://doi.org/10.1021/la0105174
State	Published - 21 Aug 2001

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abstract = "DNA hybridization to peptide nucleic acid (PNA)-functionalized Au-coated tips is characterized by probing the force interactions between the PNA-functionalized tip or the DNA/PNA-functionalized tip with a hydrophobic 1-mercaptoundecane-modified Au surface, using chemical force microscopy (CFM). DNA concentrations as low as 3 × 10-13 M can be detected by probing the respective force interactions. The CFM method is sufficiently sensitive to detect a single base mismatch in the analyte DNA.",

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AU - Patolsky, Fernando

AU - Willner, Itamar

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N2 - DNA hybridization to peptide nucleic acid (PNA)-functionalized Au-coated tips is characterized by probing the force interactions between the PNA-functionalized tip or the DNA/PNA-functionalized tip with a hydrophobic 1-mercaptoundecane-modified Au surface, using chemical force microscopy (CFM). DNA concentrations as low as 3 × 10-13 M can be detected by probing the respective force interactions. The CFM method is sufficiently sensitive to detect a single base mismatch in the analyte DNA.

AB - DNA hybridization to peptide nucleic acid (PNA)-functionalized Au-coated tips is characterized by probing the force interactions between the PNA-functionalized tip or the DNA/PNA-functionalized tip with a hydrophobic 1-mercaptoundecane-modified Au surface, using chemical force microscopy (CFM). DNA concentrations as low as 3 × 10-13 M can be detected by probing the respective force interactions. The CFM method is sufficiently sensitive to detect a single base mismatch in the analyte DNA.

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Probing of DNA and single-base mismatches by chemical force microscopy using peptide nucleic acid-modified sensing tips and functionalized surfaces

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