Probing single cells of purple sulfur bacteria with Raman spectroscopy: Carotenoids and elemental sulfur

Aharon Oren*, Lily Mana, Jan Jehlička

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

We explored the use of Raman spectroscopy to simultaneously monitor the presence of different biomarkers (carotenoids, elemental sulfur) within single cells of the purple sulfur photosynthetic bacteria Allochromatium vinosum and A. warmingii. Raman microspectrometry using excitation at 532 nm allowed the detection of different carotenoids. Raman signals of elemental sulfur appeared soon after feeding starved cells with sulfide. Raman spectroscopy is thus a convenient and sensitive technique to qualitatively and semiquantitatively assess the presence of different compounds of interest within single bacterial cells.

Original languageAmerican English
Article numberfnv021
JournalFEMS Microbiology Letters
Volume362
Issue number6
DOIs
StatePublished - 17 Feb 2015

Bibliographical note

Publisher Copyright:
© FEMS 2015. All rights reserved.

Keywords

  • Allochromatium vinosum
  • Allochromatium warmingii
  • Carotenoids
  • Purple sulfur bacteria
  • Raman spectroscopy
  • Sulfur

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